Host: HEK293 cells • AA: 62-404 • Tag: N-terminal human IgG1 Fc • MW: 65.8 kDa
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DC-SIGN1/CD209 Extracellular Domain (human, recombinant)

Item No. 38060

Product Insert (PDF)
Technical Information
Synonyms
  • CD209 Antigen Isoform 1
  • CLEC4L
  • C-type Lectin Domain Family 4 Member L
  • DC-SIGN1A Type I
  • Dendritic Cell-specific ICAM-3-grabbing Non-integrin 1
Purity
≥97% estimated by SDS-PAGE
Endotoxin Testing
<1.0 EU/µg determined by the LAL endotoxin assay
Source
Recombinant N-terminal human IgG1 Fc-tagged DC-SIGN1 extracellular domain expressed in HEK293 cells
Amino Acids
62-404
MW
65.8 kDa
Formulation (Request formulation change)
Lyophilized from sterile PBS, pH 7.4
UniProt Accession №
Q9NNX6
Shipping & Storage Information
Storage
-80°C
Shipping
Dry ice in continental US; may vary elsewhere
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    100 µg

    $328

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    1 mg

    $2,131

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    Cayman Chemical
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    Product Description

    Dendritic cell-specific ICAM-3-grabbing non-integrin 1 (DC-SIGN1), also known as CD209, is a type II transmembrane glycoprotein and member of the C-type lectin receptor superfamily.1,2 It is composed of an N-terminal cytoplasmic domain, a transmembrane domain, a neck region that contains seven repeats of 23 amino acids and is involved in oligomerization, and a carbohydrate recognition domain. However, DC-SIGN1 undergoes extensive alternative splicing that results in at least 12 membrane-bound or soluble isoforms.1,3 It is expressed in dendritic cells and macrophages, dermal, mucosal, intestine, and lung tissues, as well as blood and lymph nodes.1,2 DC-SIGN1 is involved in cell-cell adhesion and migration via interactions with intracellular cell adhesion molecule-2 (ICAM-2) and ICAM-3, recognition of viral and bacterial pathogens, and the immune response.1,2,4 It binds to HIV-1 gp120 and the receptor-binding domain of spike glycoprotein, also known as surface glycoprotein, of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2).5,6 Dendritic cell expression of human CD209 increases survival in a mouse model of M. tuberculosis infection.4 SNPs in CD209 are associated with increased disease severity in patients with dengue viral infection.7 Cayman’s DC-SIGN1/CD209 Extracellular Domain (human, recombinant) protein is a disulfide-linked homodimer. The reduced monomer, composed of DC-SIGN1 (amino acids 62-404) fused to human IgG1 Fc at its N-terminus, consists of 580 amino acids and has a calculated molecular weight of 65.8 kDa. As a result of glycosylation, the monomer migrates at approximately 75 kDa by SDS-PAGE under reducing conditions.

    WARNING This product is not for human or veterinary use.

    References & Product Citations
    Product Description References

    1. Rahimi, N. C-type lectin CD209L/L-SIGN and CD209/DC-SIGN: Cell adhesion molecules turned to pathogen recognition receptors. Biology (Basel) 10(1), 1 (2020).

    2. Geijtenbeek, T.B.H., Engering, A., and Van Kooyk, Y. DC-SIGN, a C-type lectin on dendritic cells that unveils many aspects of dendritic cell biology. J. Leukoc. Biol. 71(6), 921-931 (2002).

    3. Mummidi, S., Catano, G., Lam, L., et al. Extensive repertoire of membrane-bound and soluble dendritic cell-specific ICAM-3-grabbing nonintegrin 1 (DC-SIGN1) and DC-SIGN2 isoforms. Inter-individual variation in expression of DC-SIGN transcripts. The Journal of Biological Chemisty 276(35), 33196-33212 (2001).

    4. Schaefer, M., Reiling, N., Fessler, C., et al. Decreased pathology and prolonged survival of human DC-SIGN transgenic mice during mycobacterial infection. J. Immunol. 180(10), 6836-6845 (2008).

    5. Geijtenbeek, T.B., Kwon, D.S., Torensma, R., et al. DC-SIGN, a dendritic cell-specific HIV-1-binding protein that enhances trans-infection of T cells. Cell 100(5), 587-597 (2000).

    6. Amraei, R., Yin, W., Napoleon, M.A., et al. CD209L/L-SIGN and CD209/DC-SIGN act as receptors for SARS-CoV-2. ACS Cent. Sci. 7(7), 1156-1165 (2021).

    7. Ghosh, P., Mukherjee, S., Saha, B., et al. Importance of NFκβ, IL-10 serum levels and DC-SIGN polymorphic haplotypes in determining dengue disease severity among eastern Indian patients. Microb. Pathog. 173(Pt B), 105870 (2022).