LAMP-1/CD107a Rabbit Monoclonal Antibody (Clone 107)

Lysosome-associated membrane protein 1 (LAMP-1), also known as CD107a, is an abundant lysosomal integral membrane glycoprotein.¹ It is composed of a signal peptide, two LAMP-like domains that are separated by a proline-rich hinge region, a transmembrane domain, and a C-terminal tail that contains a YXXI tyrosine-based motif, which is required for targeting of LAMP-1 to the lysosome.^1,2 LAMP-1 is ubiquitously expressed and localizes to lysosomes, endosomes, and the plasma membrane.^1,2 It is involved in human peripheral blood mononuclear cells (PBMC) adhesion to vascular endothelium, natural killer (NK) cell degranulation, virus entry, and cancer progression and metastasis.^3,4,5,6 Homozygous knockout of Lamp1 prevents Lassa virus infection in mice.⁵ Pulmonary protein levels of LAMP-1 are increased in patients with chronic obstructive pulmonary disease (COPD), and increased LAMP-1 lung levels positively correlate with smoking history and negatively correlate with lung function.⁷ Cayman’s LAMP-1/CD107a Rabbit Monoclonal Antibody (Clone 107) can be used for ELISA, flow cytometry (FC), immunocytochemistry (ICC), immunofluorescence (IF), immunohistochemistry paraffin (IHC-P), immunoprecipitation (IP), and Western blot (WB) applications. The antibody recognizes LAMP-1 at 120 kDa from human samples.

WARNING This product is not for human or veterinary use.

1. Carlsson, S.R., and Fukuda, M. Structure of human lysosomal membrane glycoprotein 1. Assignment of disulfide bonds and visualization of its domain arrangement. The Journal of Biological Chemisty 264(34), 20526-20531 (1989).

2. Rohrer, J., Schweizer, A., Russel, D., et al. The targeting of lamp1 to lysosomes is dependent on the spacing of its cytoplasmic tail tyrosine sorting motif relative to the membrane. J. Cell Biol. 132(4), 565-576 (1996).

3. Kannan, K., Stewart, R.M., Bounds, W., et al. Lysosome-associated membrane proteins h-LAMP1 (CD107a) and h-LAMP2 (CD107b) are activation-dependent cell surface glycoproteins in human peripheral blood mononuclear cells which mediate cell adhesion to vascular endothelium. Cell. Immunol. 171(1), 10-19 (1996).

4. Krzewski, K., Gil-Krzewska, A., Nguyen, V., et al. LAMP1/CD107a is required for efficient perforin delivery to lytic granules and NK-cell cytotoxicity. Blood 121(23), 4672-4683 (2013).

5. Jae, L.T., Raaben, M., Herbert, A.S., et al. Lassa virus entry requires a trigger-induced receptor switch. Science 344(6191), 1506-1510 (2014).

6. Alessandrini, F., Pezzè, L., and Ciribilli, Y. LAMPs: Shedding light on cancer biology. Semin. Oncol. 44(4), 239-253 (2017).

7. Eapen, M.S., Lu, W., Hackett, T.L., et al. Dysregulation of endocytic machinery and ACE2 in small airways of smokers and COPD patients can augment their susceptibility to SARS-CoV-2 (COVID-19) infections. Am. J. Physiol. Lung Cell. Mol. Physiol. 320(1), L158-L163 (2020).