M13 Monoclonal Antibody (Clone MM05)

M13 Bacteriophage is a filamentous F-pilus-specific bacteriophage (Ff phage), which is a long, thin virus that infects bacterial host cells.¹ It is composed of circular single-stranded DNA (ssDNA) encapsulated by one major coat protein, p8, and four minor coat proteins, p3, p6, p7, and p9, and has approximately 98.5% sequence identity with other Ff phages such as f1 and fd.^2,1 The p8 protein accounts for approximately 85% of the total virion mass, whereas p3, p6, p7, and p9, collectively account for approximately 3% of the total virion mass.¹ Compared with f1 and fd phages, M13 bacteriophage coat proteins only differ by one amino acid at position 35 in the p8 protein. The M13 bacteriophage p8 protein is composed of an N-terminal segment that constitutes the external virion surface, a transmembrane segment, and a positively charged C-terminal segment that interacts with the ssDNA. M13 bacteriophage has commonly been used for molecular cloning, peptide phage display technology, microorganism detection, including Salmonella, cell imaging, and drug delivery.^3,2,4,1 Cayman’s recombinant M13 Monoclonal Antibody can be used for ELISA.

WARNING This product is not for human or veterinary use.

1. Han, S.M., Lee, Y.J., Lee, M.H., et al. M13 bacteriophage-based bio-nano systems for bioapplication. Biochip J. 16, 227-245 (2022).

2. Sidhu, S.S. Engineering M13 for phage display. Biomol. Eng. 18(2), 57-63 (2011).

3. Sambrook, J., and Russell, D.W. Molecular cloning: A laboratory manual. 3, 1-21 (2001).

4. Moon, J.-S., Choi, E.J., Jeong, N.-N., et al. Research progress of M13 bacteriophage-based biosensors. Nanomaterials (Basel) 9(10), 1448 (2019).