CD63 Rabbit Monoclonal Antibody (Clone 142)

CD63 is a cell surface transmembrane glycoprotein and member of the tetraspanin family.^1,2 It is composed of an intracellular N-terminal domain, a small extracellular loop, a small intracellular loop, and a large extracellular loop in between four transmembrane domains, and an intracellular C-terminal tail that contains a YXXX tyrosine-based motif, which is required for targeting of CD63 to the lysosome. CD63 is ubiquitously expressed and localizes to the plasma membrane and late endosomes, lysosomes, MHC class II compartments, and multivesicular bodies (MVBs) following clathrin-dependent endocytosis.² It is involved in cell adhesion, inflammation, platelet activation, angiogenesis, and virus entry, acts as a functional receptor for tissue inhibitor of metalloproteinases 1 (TIMP-1), and associates with various integrins.^1,3,4 Knockdown of CD63 prevents infection of human papillomavirus (HPV) and HIV-1, and antibodies targeting CD63 reduce infection of severe acute respiratory syndrome coronavirus (SARS-CoV) and Middle East respiratory syndrome coronavirus (MERS-CoV) in vitro.³ Protein levels of CD63 are increased in patients with esophageal carcinoma and negatively correlate with tumor stage and lymph node metastasis.⁵ Cayman's CD63 Rabbit Monoclonal Antibody (Clone 142) can be used for ELISA.

WARNING This product is not for human or veterinary use.

1. Justo, B.L., and Jasiulionis, M.G. Characteristics of TIMP1, CD63, and β1-integrin and the functional impact of their interaction in cancer. Int. J. Mol. Sci. 22(17), 9319 (2021).

2. Pols, M.S., and Klumperman, J. Trafficking and function of the tetraspanin CD63. Exp. Cell Res. 315(9), 1584-1592 (2009).

3. New, C., Lee, Z.-Y., Tan, K.S., et al. Tetraspanins: Host factors in viral infections. Int. J. Mol. Sci. 22(21), 11609 (2021).

4. Maecker, H.T., Todd, S.C., and Levy, S. The tetraspanin superfamily: Molecular facilitators. The FASEB Journal 11(6), 428-442 (1997).

5. Lai, X.P., Gu, Q., Zhou, X., et al. Decreased expression of CD63 tetraspanin protein predicts elevated malignant potential in human esophageal cancer. Oncol. Lett. 13(6), 4245-4251 (2017).