EPCR/CD201 Rabbit Monoclonal Antibody (Clone 041)

Endothelial protein C receptor (EPCR), also known as CD201, is a type I transmembrane glycoprotein involved in coagulation and hemostasis, cytoprotection, and innate immunity.¹ It is composed of a signal peptide, α1 and α2 domains, which share homology with CD1d and bind to phosphatidylcholine, a transmembrane domain, and a cytoplasmic tail.^2,3 EPCR is expressed on the surface of endothelial cells and can be cleaved by disintegrin and metalloproteinase domain-containing protein 17 (ADAM17/TACE) to release soluble EPCR, which is a process known as shedding and is induced by thrombin and pro-inflammatory mediators, including IL-1β, TNF-α, and phorbol esters.^3,4 Binding of protein C to EPCR facilitates its activation by the thrombin-thrombomodulin complex, which can then inactivate factor Va and factor VIIIa to promote anticoagulation, and EPCR-associated activated protein C cleaves proteinase-activated receptor 1 (PAR1) to initiate cytoprotective signaling.³ EPCR is also involved in inflammation, malaria pathogenesis, and cancer metastasis.¹ Knockout of EPCR protects against needle-induced joint bleeding in a mouse model of hemophilia A.⁵ SNPs in EPCR are associated with venous thromboembolism (VTE).⁶ Cayman’s EPCR/CD201 Rabbit Monoclonal Antibody (Clone 041) can be used for immunohistochemistry paraffin (IHC-P).

WARNING This product is not for human or veterinary use.

1. Mohan Rao, L.V., Esmon, C.T., and Pendurthi, U.R. Endothelial cell protein C receptor: A multiliganded and multifunctional receptor. Blood 124(10), 1553-1562 (2014).

2. Simmonds, R.E., and Lane, D.A. Structural and functional implications of the intron/exon organization of the human endothelial cell protein C/activated protein C receptor (EPCR) gene: Comparison with the structure of CD1/major histocompatibility complex α1 and α2 domains. Blood 94(2), 632-641 (1999).

3. Bouwens, E.A.M., Stavenuiter, F., and Mosnier, L.O. Mechanisms of anticoagulant and cytoprotective actions of the protein C pathway. J. Thromb. Haemost. 11(Suppl. 1), 242-253 (2013).

4. Fukudome, K., and Esmon, C.T. Identification, cloning, and regulation of a novel endothelial cell protein C/activated protein C receptor. The Journal of Biological Chemisty 269(42), 26486-26491 (1994).

5. Magisetty, J., Pendurthi, U.R., Esmon, C.T., et al. EPCR deficiency or function-blocking antibody protects against joint bleeding-induced pathology in hemophilia mice. Blood 135(25), 2211-2223 (2020).

6. Dennis, J., Johnson, C.Y., Adediran, A.S., et al. The endothelial protein C receptor (PROCR) Ser219Gly variant and risk of common thrombotic disorders: A HuGE review and meta-analysis of evidence from observational studies. Blood 119(10), 2392-2400 (2012).