LFA-3/CD58 Rabbit Monoclonal Antibody (Clone 126)

Lymphocyte function-associated antigen 3 (LFA-3), also known as CD58, is a membrane-associated glycoprotein.¹ It exists as two isoforms: a type I transmembrane glycoprotein involved in signal transduction and a glycosylphosphatidylinositol-anchored (GPI-anchored) form primarily involved in cell adhesion.^1,2 A soluble form of LFA-3, sCD58, also exists.¹ Transmembrane LFA-3 is composed of an N-terminal extracellular domain containing N-linked glycosylation sites, a transmembrane domain, and a short C-terminal cytoplasmic tail. The GPI-anchored form is located extracellularly and is linked to the plasma membrane via the C-terminus. LFA-3 is expressed broadly on hematopoietic and non-hematopoietic cells and its ligand, CD2, is expressed on T cells, natural killer (NK) cells, thymocytes, and certain bone marrow cells. LFA-3 acts as a co-stimulatory receptor that, when bound by CD2 on the surface of T cells, is part of the immunological synapse and induces T cell activation. It is also involved in thymocyte development and NK cell communication, as well as other immune and non-immune functions. sCD58 disrupts cell-cell adhesion and has immunosuppressive effects. Various SNPs in CD58 are associated with an increased risk of multiple sclerosis with one SNP potentially conferring a protective effect.^3,4 The expression of CD58 is increased or decreased in various cancers with increased expression positively correlated with overall survival and disease-free survival in patients with acute myeloid leukemia (AML).¹ Cayman’s LFA-3/CD58 Rabbit Monoclonal Antibody (Clone 126) can be used for immunohistochemistry paraffin (IHC-P).

WARNING This product is not for human or veterinary use.

1. Zhang, Y., Liu, Q., Yang, S., et al. CD58 immunobiology at a glance. Front. Immunol. 12, 705260 (2021).

2. Dustin, M.L., Selvaraj, P., Mattaliano, R.J., et al. Anchoring mechanisms for LFA-3 cell adhesion glycoprotein at membrane surface. Nature 329(6142), 846-848 (1987).

3. Hecker, M., Fitzner, B., Blaschke, J., et al. Susceptibility variants in the CD58 gene locus point to a role of microRNA-548ac in the pathogenesis of multiple sclerosis. Mutat. Res. Rev. Mutat. Res. 763, 161-167 (2015).

4. De Jager, P.L., Baecher-Allan, C., Maier, L.M., et al. The role of the CD58 locus in multiple sclerosis. Proc. Natl. Acad. Sci. USA 106(13), 5264-5269 (2009).