FABP4 Rabbit Monoclonal Antibody (Clone 001)

Fatty acid binding protein 4 (FABP4) is a member of the intracellular lipid-binding protein (iLBP) family.¹ It is composed of a β-barrel, which forms the lipid-binding site, and two α-helices that form a lid on the β-barrel.² FABP4 is primarily expressed in adipocytes but has also been found in activated macrophages.^3,4 It is involved in lipid transport and metabolism, insulin resistance, and angiogenesis, and its expression is induced by long-chain fatty acids and peroxisome proliferator-activated receptor γ (PPARγ) agonists.^1,5,6,7,8 Knockout of Fabp4 prevents the development of atherosclerosis in ApoE^-/- mice and increases body weight gain and insulin sensitivity in ob/ob mice.^9,5 Serum levels of FABP4 are increased in patients with colorectal cancer, and serum and synovial fluid levels of FABP4 are increased in patients with rheumatoid arthritis.^10,7 Cayman’s FABP4 Rabbit Monoclonal Antibody (Clone 001) can be used for flow cytometry (FC) and immunohistochemistry-paraffin (IHC-P) applications.

WARNING This product is not for human or veterinary use.

1. Zimmerman, A.W., and Veerkamp, J.H. New insights into the structure and function of fatty acid-binding proteins. Cell. Mol. Life Sci. 59(7), 1096-1116 (2002).

2. Reese-Wagoner, A., Thompson, J., and Banaszak, L. Structural properties of the adipocyte lipid binding protein. Biochim. Biophys. Acta 1441(2-3), 106-116 (1999).

3. Baxa, C.A., Sha, R.S., Buelt, M.K., et al. Human adipocyte lipid-binding protein: Purification of the protein and cloning of its complementary DNA. Biochemistry 28(22), 8683-8690 (1989).

4. Perrella, M.A., Pellacani, A., Layne, M.D., et al. Absence of adipocyte fatty acid binding protein prevents the development of accelerated atherosclerosis in hypercholesterolemic mice. The FASEB Journal 15(10), 1774-1776 (2001).

5. Uysal, K.T., Scheja, L., Wiesbrock, S.M., et al. Improved glucose and lipid metabolism in genetically obese mice lacking aP2. Endocrinology 141(9), 3388-3396 (2000).

6. Hotamisligil, G.S., Johnson, R.S., Distel, R.J., et al. Uncoupling of obesity from insulin resistance through a targeted mutation in aP2, the adipocyte fatty acid binding protein. Science 274(5291), 1377-1379 (1996).

7. Guo, D., Lin, C., Lu, Y., et al. FABP4 secreted by M1-polarized macrophages promotes synovitis and angiogenesis to exacerbate rheumatoid arthritis. Bone Res. 10(1), 45 (2022).

8. Bastie, C., Holst, D., Gaillard, D., et al. Expression of preoxisome proliferator-activated receptor PPARδ promotes induction of PPARγ and adipocyte differentiation in 3T3C2 fibroblasts. The Journal of Biological Chemisty 274(31), 21920-21925 (1999).

9. Lim, G.P., Yang, F., Chu, T., et al. Ibuprofen suppresses plaque pathology and inflammation in a mouse model for Alzheimer’s disease. J. Neurosci. 20(15), 5709-5714 (2000).

10. Zhang, Y., Zhao, X., Deng, L., et al. High expression of FABP4 and FABP6 in patients with colorectal cancer. World J. Surg. Oncol. 17(1), 171 (2019).