For immunochemical detection of IL-6Rα
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Interleukin-6Rα/CD126 Rabbit Monoclonal Antibody (Clone 033)

Item No. 38089

Product Insert (PDF)
Technical Information
Synonyms
  • IL-6Rα
  • Interleukin-6 Receptor Subunit α
Immunogen
Recombinant mouse IL-6Rα
Clone Designation
033
Formulation (Request formulation change)
50, 100 µl, or 1 ml of protein A-affinity purified monoclonal antibody
Storage Buffer
0.2 μm filtered solution in PBS
Host
Rabbit
Isotype
IgG
Applications
ELISA (capture), FC
Cross Reactivity
(+) IL-6Rα(+) sIL-6Rα
Species Reactivity
(+) Mouse
Shipping & Storage Information
Storage
-80°C
Shipping
Dry ice in continental US; may vary elsewhere
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Certificates of Analysis & Batch Specific Data

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    50 µl

    $179

    Add

    100 µl

    $285

    Add

    1 ml

    $1,782

    Add
    Cayman Chemical
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    Product Description

    Interleukin-6Rα (IL-6Rα), also known as CD126, is a type I cytokine receptor.1 It is composed of an immunoglobulin-like domain, a cytokine-binding domain, a transmembrane domain, and a cytoplasmic domain and forms a heteromeric complex with IL-6 and gp130, which is the signal transducing subunit of the IL-6R that primarily acts through the JAK/STAT3 pathway.2 IL-6Rα is expressed in hepatocytes and monocytes and is localized to the plasma membrane but also exists as a soluble form (sIL-6Rα) that is primarily generated via proteolytic cleavage of membrane-bound IL-6Rα, but can also be formed via alternative splicing.3,4,5 Binding of IL-6 to membrane-bound IL-6Rα, known as classical signaling, upregulates the synthesis of acute phase proteins, induces lipolysis in the liver, and induces the differentiation of T and B cells and is primarily anti-inflammatory.6,7 Trans-signaling consists of IL-6 binding to sIL-6R, which has various pro-inflammatory effects. Knockdown of IL6R increases sensitivity to tamoxifen in breast cancer cells.8 Formulations containing chimeric anti-IL-6Rα monoclonal antibodies have been used in the treatment of rheumatoid arthritis, idiopathic arthritis, giant cell arteritis, cytokine release syndrome, and COVID-19. Cayman's Interleukin-6Rα/CD126 Rabbit Monoclonal Antibody (Clone 033) can be used for ELISA (capture) and flow cytometry (FC) applications.

    WARNING This product is not for human or veterinary use.

    References & Product Citations
    Product Description References

    1. Hirano, T. Interleukin 6 and its receptor: Ten years later. Int. Rev. Immunol. 16(3-4), 249-284 (1998).

    2. Salvati, A.L., Lahm, A., Paonessa, G., et al. Interleukin-6 (IL-6) antagonism by soluble IL-6 receptor α mutated in the predicted gp130-binding interface. The Journal of Biological Chemisty 270(20), 12242-12249 (1995).

    3. Bauer, J., Lengyel, G., Bauer, T.M., et al. Regulation of interleukin-6 receptor expression in human monocytes and hepatocytes. FEBS Lett. 249(1), 27-30 (1989).

    4. Flynn, C.M., Kespohl, B., Daunke, T., et al. Interleukin-6 controls recycling and degradation, but not internalization of its receptors. The Journal of Biological Chemisty 296, 100434 (2021).

    5. Riethmueller, S., Somasundaram, P., Ehlers, J.C., et al. Proteolytic origin of the soluble human IL-6R in vivo and a decisive role of N-glycosylation. PLoS Biol. 15(1), e2000080 (2017).

    6. Del Giudice, M., and Gangestad, S.W. Rethinking IL-6 and CRP: Why they are more than inflammatory biomarkers, and why it matters. Brain Behav. Immun. 70, 61-75 (2018).

    7. Rose-John, S. Interleukin-6 family cytokines. Cold Spring Harb. Perspect. Biol. 10(2), a028415 (2018).

    8. Tsoi, H., Man, E.P.S., Chau, K.M., et al. Targeting the IL-6/STAT3 signalling cascade to reverse tamoxifen resistance in estrogen receptor positive breast cancer. Cancers (Basel) 13(7), 1511 (2021).