For immunochemical detection of FcγRIIIa
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FcγRIIIa/CD16a Polyclonal Antibody

Item No. 38098

Product Insert (PDF)Safety Data Sheet (SDS) (PDF)
Technical Information
Synonyms
  • FCG3
  • FCGR3A
  • FCR-10
  • FCRIIIa
  • FGR3A
Immunogen
Recombinant human CD16a
Formulation (Request formulation change)
50, 100, or 200 µl of protein A-affinity purified polyclonal antibody
Storage Buffer
0.2 μm filtered solution in PBS
Host
Rabbit
Isotype
IgG
Applications
ELISA, IHC-P
Cross Reactivity
(+) FcγRIIIa
Species Reactivity
(+) Human(+) Cynomolgus monkey
Shipping & Storage Information
Storage
-80°C
Shipping
Dry ice in continental US; may vary elsewhere
Recommended Products

Certificates of Analysis & Batch Specific Data

Provide batch numbers separated by commas to download or request available product inserts, QC sheets, certificates of analysis, data packs, and GC-MS data.

    50 µl

    $179

    Add

    100 µl

    $268

    Add

    200 µl

    $446

    Add
    Cayman Chemical
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    Product Description

    Fc γ receptor IIIa (FcγRIIIa), also known as CD16a, is an activating receptor for IgG with roles in antibody-dependent cell cytotoxicity (ADCC).1,2 It is expressed primarily in natural killer (NK) cells, but also in macrophages and non-classical monocytes.2,3 FcγRIIIa is composed of an extracellular domain which contains five N-glycosylation sites, a transmembrane helix, and a cytoplasmic tail.2 Upon binding of IgG, FcγRIIIa engages one of two signaling chains, CD3ζ or FcεRIγ, which is phosphorylated by the Src family kinase LCK and induces intracellular signaling and neutrophil activation.1 FcγRIIIa signaling is positively correlated with disease progression and pathogenic lung inflammation in patients with COVID-19.3 Mutations in FCGR3A, the gene encoding FcγRIIIa, are positively correlated with increased risk of colorectal carcinoma.4 Cayman’s FcγRIIIa/CD16a Polyclonal Antibody can be used for ELISA and immunohistochemistry-paraffin (IHC-P) applications.

    WARNING This product is not for human or veterinary use.

    References & Product Citations
    Product Description References

    1. Coënon, L., and Villalba, M. From CD16a biology to antibody-dependent cell-mediated cytotoxicity improvement. Front. Immunol. 13, 913215 (2022).

    2. Patel, K.R., Benavente, M.C.R., Lorenz, W.W., et al. Fc γ receptor IIIa/CD16a processing correlates with the expression of glycan-related genes in human natural killer cells. The Journal of Biological Chemisty 296, 100183 (2021).

    3. Gonzalez, J.C., Chakraborty, S., Thulin, N.K., et al. Heterogeneity in IgG-CD16 signaling in infectious disease outcomes. Immunol. Rev. 309(1), 64-74 (2022).

    4. Canossi, A., Aureli, A., Del Beato, T., et al. Role of KIR and CD16A genotypes in colorectal carcinoma genetic risk and clinical stage. J. Transl. Med. 14(1), 239 (2016).