For immunochemical detection of KLH
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KLH Monoclonal Antibody (Clone 15F7E4G3)

Item No. 38099

Product Insert (PDF)Safety Data Sheet (SDS) (PDF)
Technical Information
Synonyms
  • Keyhole Limpet Hemocyanin
Immunogen
Recombinant KLH
Clone Designation
15F7E4G3
Formulation (Request formulation change)
50, 100, or 200 µl of protein A-affinity purified monoclonal antibody
Storage Buffer
0.2 μm filtered solution in PBS
Host
Mouse
Isotype
IgG1
Applications
ELISA, WB
Cross Reactivity
(+) KLH
Shipping & Storage Information
Storage
-80°C
Shipping
Dry ice in continental US; may vary elsewhere
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Certificates of Analysis & Batch Specific Data

Provide batch numbers separated by commas to download or request available product inserts, QC sheets, certificates of analysis, data packs, and GC-MS data.

    50 µl

    $179

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    100 µl

    $268

    Add

    200 µl

    $428

    Add
    Cayman Chemical
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    Product Description

    Keyhole limpet hemocyanin (KLH) is a copper-containing respiratory glycoprotein from the hemolymph of the marine mollusk M. crenulata that is involved in oxygen uptake, transport, and release.1,2,3 It exists as two isoforms, KLH1 and KLH2, and is composed of eight globular functional units that each contain an oxygen binding site and arrange into decamers, didecamers, or multidecamers to form a cylindrical pore.1,4 Due to its immunogenicity, KLH has commonly been used as a T cell-dependent model antigen.2,5 Immunization of mice with a peptide derived from A. baumannii outer membrane protein A (OmpA) conjugated to KLH produces a monoclonal antibody that increases macrophage opsonization of antibiotic-resistant A. baumannii in vitro.6 Serum isolated from mice vaccinated with KLH conjugated to 2,4-dinitrophenyl (DNP) and an analog of the tumor-associated carbohydrate antigen ganglioside GM3 exhibits increased IgG antibody titers and induces antibody-dependent cell cytotoxicity (ADCC) in B16/F10 mouse skin melanoma cells expressing a DNP-ganglioside GM3 analog conjugate.7 Cayman’s KLH Monoclonal Antibody (Clone 15F7E4G3) can be used for ELISA and Western blot (WB) applications.

    WARNING This product is not for human or veterinary use.

    References & Product Citations
    Product Description References

    1. Swerdlow, R.D., Ebert, R.F., Lee, P., et al. Keyhole limpet hemocyanin: Structural and functional characterization of two different subunits and multimers. Comp. Biochem. Physiol. B Biochem. Mol. Biol. 113(3), 537-548 (1996).

    2. Harris, J.R., and Markl, J. Keyhole limpet hemocyanin (KLH): A biomedical review. Micron 30(6), 597-623 (1999).

    3. Kurokawa, T., Wuhrer, M., Lochnit, G., et al. Hemocyanin from the keyhole limpet Megathura crenulata (KLH) carries a novel type of N-glycans with Gal(β1-6)Man-motifs. Eur. J. Biochem. 269(22), 5459-5473 (2002).

    4. Gastogiannis, C., and Markl, J. Keyhole limpet hemocyanin: 9-A CryoEM structure and molecular model of the KLH1 didecamer reveal the interfaces and intricate topology of the 160 functional units. J. Mol. Biol. 385(3), 963-983 (2009).

    5. Swaminathan, A., Lucas, R.M., Dear, K., et al. Keyhole limpet haemocyanin - A model antigen for human immunotoxicological studies. Br. J. Clin. Pharmac. 78(5), 1135-1142 (2014).

    6. Yeganeh, O., Shabani, M., Pakzad, P., et al. Evaluation the reactivity of a peptide-based monoclonal antibody derived from OmpA with drug resistant pulsotypes of Acinetobacter baumannii as a potential therapeutic approach. Ann. Clin. Microbiol. Antimicrob. 21(1), 30 (2022).

    7. Lin, H., Hong, H., Feng, L., et al. Synthesis of DNP-modified GM3-based anticancer vaccine and evaluation of its immunological activities for cancer immunotherapy. Chin. Chem. Lett. 32(12), 4041-4044 (2021).