For immunochemical detection of CD8a
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CD8a Polyclonal Antibody

Item No. 38101

Product Insert (PDF)Safety Data Sheet (SDS) (PDF)
Technical Information
Synonyms
  • T Cell Surface Glycoprotein CD8 α-chain
  • T Cell Surface Glycoprotein CD8 Alpha Chain
Immunogen
Recombinant mouse CD8a
Formulation (Request formulation change)
50, 100, or 200 µl of protein A-affinity purified polyclonal antibody
Storage Buffer
0.2 μm filtered solution in PBS
Host
Rabbit
Isotype
IgG
Applications
ELISA, ICC, IF, IHC-P
Cross Reactivity
(+) CD8a
Species Reactivity
(+) Mouse
Shipping & Storage Information
Storage
-80°C
Shipping
Dry ice in continental US; may vary elsewhere
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Certificates of Analysis & Batch Specific Data

Provide batch numbers separated by commas to download or request available product inserts, QC sheets, certificates of analysis, data packs, and GC-MS data.

    50 µl

    $179

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    100 µl

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    200 µl

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    Cayman Chemical
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    Product Description

    The CD8 α-chain (CD8a) is a transmembrane glycoprotein that exists as a homodimer, CD8αα, or heterodimer with the CD8 β-chain (CD8b), CD8αβ, to form the T cell receptor (TCR) co-receptor CD8.1,2 CD8a is composed of an extracellular immunoglobulin variable-like (IgV) domain, which interacts with MHC class I molecules, a transmembrane domain, and a cytoplasmic tail that associates with the tyrosine kinase p56Ick and mediates signal transduction.1,3,2 CD8αβ is expressed by cytotoxic T lymphocytes (CTLs), where it acts as a TCR co-receptor by enhancing T cell signaling and activation upon binding to antigen-displaying MHC class I molecules.4,2 CD8αα is expressed by CTLs and intestinal epithelial lymphocytes, as well as γδ T, natural killer (NK), and dendritic cells, where it acts as a positive or negative regulator of cell activation in a cell-dependent manner. Cd8a-/- mice infected with M. tuberculosis exhibit reduced survival.5 Cayman's CD8a Polyclonal Antibody can be used for ELISA, immunocytochemistry (ICC), immunofluorescence (IF), and immunohistochemistry (IHC; paraffin) applications.

    WARNING This product is not for human or veterinary use.

    References & Product Citations
    Product Description References

    1. Laugel, B., Cole, D.K., Clement, M., et al. The multiple roles of the CD8 coreceptor in T cell biology: Opportunities for the selective modulation of self-reactive cytotoxic T cells. J. Leukoc. Biol. 90(6), 1089-1099 (2011).

    2. Cheroutre, H., and Lambolez, F. Doubting the TCR coreceptor function of CD8αα. Immunity 28(2), 149-159 (2008).

    3. Irie, H.Y., Ravichandran, K.S., and Burakoff, S.J. CD8β chain influences CD8α chain-associated Lck kinase activity. J. Exp. Med. 181(4), 1267-1273 (1995).

    4. Geng, J., and Raghavan, M. CD8αα homodimers function as a coreceptor for KIR3DL1. Proc. Natl. Acad. Sci. USA 115(36), 17951-17956 (2019).

    5. Sousa, A.O., Mazzaccaro, R.J., Russell, R.G., et al. Relative contributions of distinct MHC class I-dependent cell populations in protection to tuberculosis infection in mice. Proc. Natl. Acad. Sci. USA 97(8), 4204-4208 (2000).