For immunoprecipitation of MR1
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MR1 Immunoaffinity Sorbent

Item No. 38215

Product Insert (PDF)Safety Data Sheet (SDS) (PDF)
Technical Information
Synonyms
  • Class I Histocompatibility Antigen-like Protein
  • Major Histocompatibility Complex Class I-related Gene Protein
  • MHC Class I-related Gene Protein
  • ​MR1 Immunoaffinity Beads
  • MR1 Immunoaffinity Resins
Immunogen
Recombinant human MR1 (aa 22-292) protein
Clone Designation
2H7
Formulation
400 µl of CNBr-activated Sepharose 4B coupled to a human MR1 monoclonal antibody (Clone 2H7) (Item No. 34257)
Storage Buffer
PBS, pH 7.2, with 0.02% sodium azide
Host
Mouse
Isotype
IgG1
Applications
IP, WB, LC-MS
Cross Reactivity
(+) MR1
Species Reactivity
(+) Human
UniProt Accession №
Q95460
Shipping & Storage Information
Storage
4°C
Shipping
Wet ice in continental US; may vary elsewhere
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    400 µl

    $448

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    Cayman Chemical
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    Product Description

    Major histocompatibility complex (MHC) class I-related gene protein (MR1) is a non-polymorphic MHC class Ib antigen-presenting cell surface molecule that is required for T cell receptor-mediated activation of mucosal-associated invariant T (MAIT) cells.1,2 It is composed of α1 and α2 domains, which form an antigen-binding pocket, and an α3 domain that interacts with β2-microglobulin.2 MR1 mRNA is ubiquitously expressed and, following translation, MR1 protein is localized to the endoplasmic reticulum in a partially folded state. Upon binding of a riboflavin-derived microbial antigen, MR1 undergoes a conformational change and translocates to the cell surface where it induces MAIT cell activation via an interaction with the MAIT cell T cell receptor and activates various immunomodulatory effects, including cytokine release, initiation of adaptive immune responses, and promotion of tissue repair.3 Cayman's MR1 Immunoaffinity Sorbent is designed for immunoprecipitation (IP) of MR1 protein from biological samples. This is an effective way to concentrate MR1 protein for subsequent detection by a different MR1 antibody by immunoprecipitation (IP) (LC/MS certified) and Western blot (WB). The MR1 affinity sorbent consists of Cayman's MR1 Monoclonal Antibody (Clone 2H7) (Item No. 34257) coupled to CNBr-activated Sepharose 4B.

    WARNING This product is not for human or veterinary use.

    References & Product Citations
    Product Description References

    1. Lamichhane, R., and Ussher, J.E. Expression and trafficking of MR1. Immunology 151(3), 270-279 (2017).

    2. Krovi, S.H., and Gapin, L. Structure and function of the non-classical major histocompatibility complex molecule MR1. Immunogenetics 68(8), 549-559 (2016).

    3. McWilliam, H.E.G., and Salio, M. Understanding and modulating the MR1 metabolite antigen presentation pathway. Mol. Immunol. 129, 121-126 (2021).