Active • Host: E. coli • AA: 36-714 • Tag: C-terminal His • MW: 76.2 kDa
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Product Categories

Product Type

Proteins
Enzymes
Hydrolases
Synthases

Research Area

Lipid Biochemistry
Sphingolipids

Application

Enzyme Activity

SCDase (Shewanella alga, recombinant)

Item No. 38256

Product Insert (PDF)Safety Data Sheet (SDS) (PDF)
Technical Information
Synonyms
  • Ricin-type β-trefoil Lectin Domain Protein
  • Sphingolipid Ceramide N-deacylase
Purity
≥50% estimated by SDS-PAGE
Source
Active recombinant S. alga C-terminal His-tagged SCDase expressed in E. coli
Amino Acids
36-714
MW
76.2 kDa
Formulation (Request formulation change)
50 mM sodium acetate, pH 6.0, with 0.1% Triton X-100 and 1 mM calcium chloride
UniProt Accession №
NCBI Reference Sequence WP_144223911.1
Shipping & Storage Information
Storage
-80°C
Shipping
Dry ice in continental US; may vary elsewhere
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    Product Description

    Sphingolipid ceramide N-deacylase (SCDase) is an enzyme that catalyzes the hydrolysis or synthesis of the N-acyl linkage between the fatty acyl chain and sphingosine base in glycosphingolipids and sphingomyelin but not in free ceramide.1,2 It has a broad substrate specificity for both hydrolysis and synthesis with preferences for glycosphingolipids with large sugar moieties and charged head groups for the hydrolysis reaction and glycosphingolipids with more polar head groups for the synthesis reaction. SCDase has been used as a biocatalyst for the synthesis and hydrolysis of glycosphingolipids.1,3 Cayman’s SCDase (Shewanella alga, recombinant) protein can be used for bioconversion and enzyme activity assay applications.

    WARNING This product is not for human or veterinary use.

    References & Product Citations
    Product Description References

    1. Han, Y.-B., Wu, L., Rich, J.R., et al. Comprehensive characterization of sphingolipid ceramide N-deacylase for the synthesis and fatty acid remodeling of glycosphingolipids. Appl. Microbiol. Biotechnol. 99(16), 6715-6726 (2015).

    2. Ito, M., Kurita, T., and Kita, K. A novel enzyme that cleaves the N-acyl linkage of ceramides in various glycosphingolipids as well as sphingomyelin to produce their lyso forms. The Journal of Biological Chemisty 270(41), 24370-24374 (1995).

    3. Chakrabertyk, R., Reiz, B., and Cairo, C.W. Profiling of glycosphingolipids with SCDase digestion and HPLC-FLD-MS. Anal. Biochem. 631, 114361 (2021).