A fluorescent probe for NAD+-dependent enzymes
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ε-NAD+ (sodium salt)

Item No. 38385

Product Insert (PDF)Safety Data Sheet (SDS) (PDF)
Technical Information
Formal Name
3-(aminocarbonyl)-1-[5-O-[hydroxy(phosphonooxy)phosphinyl]-β-D-ribofuranosyl]-pyridinium, P′→5′-ester with 3-β-D-ribofuranosyl-3H-imidazo[2,1-i]purine, inner salt, monosodium salt
Synonyms
  • N6-Ethenoadenine dinucleotide
  • β-Nicotinamide-1
  • ε-Nicotinamide adenine dinucleotide
Molecular Formula
C23H26N7O14P2 • Na
Formula Weight
709.4
Purity
≥95%
Emission
410 nm
Excitation
295-300 nm
Formulation (Request formulation change)
A solid
Solubility(Learn about Variance in Solubility)
Water: Soluble
SMILES
O=C(N)C1=CC=C[N+]([C@H]2[C@H](O)[C@H](O)[C@@H](COP([O-])(OP(OC[C@H]3O[C@@H](N4C=NC5=C4N=CN6C5=NC=C6)[C@H](O)[C@@H]3O)([O-])=O)=O)O2)=C1.[Na+]
InChi Code
InChI=1S/C23H27N7O14P2.Na/c24-19(35)11-2-1-4-28(6-11)22-17(33)15(31)12(42-22)7-40-45(36,37)44-46(38,39)41-8-13-16(32)18(34)23(43-13)30-10-26-14-20-25-3-5-29(20)9-27-21(14)30;/h1-6,9-10,12-13,15-18,22-23,31-34H,7-8H2,(H3-,24,35,36,37,38,39);/q;+1/p-1/t12-,13-,15-,16-,17-,18-,22-,23-;/m1./s1
InChi Key
DMGKZKQWRUKHHF-JIVSRYSWSA-M
Shipping & Storage Information
Storage
-80°C
Shipping
Dry ice in continental US; may vary elsewhere
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    6.9 mg

    $224

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    Product Description

    ε-NAD+ is a fluorescent probe for enzymes that use the signaling molecule and enzyme cofactor NAD+ (Item No. 16077), such as glutamate dehydrogenase (GDH) or alcohol dehydrogenase (ADH).1,2 Upon enzymatic cleavage, 5'-ε-AMP is released and its fluorescence can be used to quantify enzyme activity. ε-NAD+ displays an emission maximum of 410 nm upon excitation at 295-300 nm.2

    WARNING This product is not for human or veterinary use.

    References & Product Citations
    Product Description References

    1. Favilla, R., and Mazzini, A. The binding of 1,N6-etheno-NAD to bovine liver glutamate dehydrogenase. Biochim. Biophys. Acta 788(1), 48-57 (1984).

    2. Barrio, J.R., Secrist, J.A., 3rd., and Leonard, N.J. A fluorescent analog of nicotinamide adenine dinucleotide. Proc. Natl. Acad. Sci. USA 69(8), 2039-2042 (1972).