An allosteric activator of AMPKβ1 heterotrimers
Technical Support & Resources

Information provided in the product description is from published literature. Due to the nature of scientific experimentation, your results (e.g., selectivity and effective concentrations) or specific application for this product may differ. If you have questions about how this product fits your application, please contact our technical support staff.

Visit our FAQ

Contact Us

Toll Free Phone (USA and Canada Only): (888) 526-5351
Direct Phone: (734) 975-3888

Request Technical Support

Technical Support Request

To streamline the process attach the appropriate questionnaire to your inquiry.

Download IHC QuestionnaireDownload WB Questionnaire

View Our Privacy Statement for details on how we use and protect your data. In addition, this site is protected by hCaptcha and its Privacy Policy and Terms of Service apply.

PF-249

Item No. 38416

Product Insert (PDF)Safety Data Sheet (SDS) (PDF)
Technical Information
Formal Name
6-chloro-5-[6-(dimethylamino)-2-methoxy-3-pyridinyl]-1H-indole-3-carboxylic acid
CAS Number
1467059-70-6
Synonyms
  • PF-06885249
Molecular Formula
C17H16ClN3O3
Formula Weight
345.8
Purity
≥98%
Formulation (Request formulation change)
A solid
Solubility(Learn about Variance in Solubility)
DMF: 10 mg/mlDMSO: 1 mg/mlEthanol: 5 mg/ml
λmax
224, 255, 301 nm
SMILES
O=C(C1=CNC2=C1C=C(C3=C(OC)N=C(N(C)C)C=C3)C(Cl)=C2)O
InChi Code
InChI=1S/C17H16ClN3O3/c1-21(2)15-5-4-9(16(20-15)24-3)10-6-11-12(17(22)23)8-19-14(11)7-13(10)18/h4-8,19H,1-3H3,(H,22,23)
InChi Key
MJHBRTGGZYSCHJ-UHFFFAOYSA-N
Shipping & Storage Information
Storage
-20°C
Shipping
Room temperature in continental US; may vary elsewhere
Recommended Products

Certificates of Analysis & Batch Specific Data

Provide batch numbers separated by commas to download or request available product inserts, QC sheets, certificates of analysis, data packs, and GC-MS data.

Download most recent Certificate of Analysis for this product

    1 mg

    $40

    Add

    5 mg

    $179

    Add

    10 mg

    $315

    Add

    25 mg

    $590

    Add
    Product Description

    PF-249 is an allosteric activator of AMP-activated protein kinase β1 (AMPKβ1) heterotrimers (EC50s = 8 and 6 nM for human AMPK α1β1γ1 and -α2β1γ1, respectively).1,2 It is selective for AMPK α1β1γ1 and -α2β1γ1 over AMPK α1β2γ1, -α2β2γ1, and -α2β2γ3 at 40 µM and a panel of receptors, channels, and phosphodiesterases at 10 µM.1,2 PF-249 decreases proteinuria and improves kidney function in an obese ZSF1 rat model of diabetic nephropathy in a dose-dependent manner.1

    WARNING This product is not for human or veterinary use.

    References & Product Citations
    Product Description References

    1. Salatto, C.T., Miller, R.A., Cameron, K.O., et al. Selective activation of AMPK β1-containing isoforms improves kidney function in a rat model of diabetic nephropathy. J. Pharmacol. Exp. Ther. 361(2), 303-311 (2017).

    2. Ryder, T.F., Calabrese, M.F., Walker, G.S., et al. Acyl glucuronide metabolites of 6-chloro-5-[4-(1-hydroxycyclobutyl)phenyl]-1H-indole-3-carboxylic acid (PF-06409577) and related indole-3-carboxylic acid derivatives are direct activators of adenosine monophosphate-activated protein kinase (AMPK). J. Med. Chem. 61(16), 7273-7288 (2018).