A hydrolysis-resistant analog of GTP
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Guanosine 5'-[γ-thio]triphosphate (sodium salt)

Item No. 38565

Product Insert (PDF)Safety Data Sheet (SDS) (PDF)
Technical Information
Formal Name
guanosine 5′-(trihydrogen diphosphate), P′-anhydride with phosphorothioic acid, tetrasodium salt
CAS Number
1486471-09-3
Synonyms
  • GTPγS
  • Guanosine 5'-O-(3-thio)triphosphate
  • Guanosine 5'-trihydrogen diphosphate monoanhydride Phosphorothioic Acid
Molecular Formula
C10H12N5O13P3S • 4Na
Formula Weight
627.2
Purity
≥95%
Formulation (Request formulation change)
A 10 mM solution in water
Solubility(Learn about Variance in Solubility)
Water: Soluble
SMILES
O[C@@H]1[C@H](O)[C@@H](COP([O-])(OP(OP([O-])([S-])=O)([O-])=O)=O)O[C@H]1N2C=NC3=C2NC(N)=NC3=O.[Na+].[Na+].[Na+].[Na+]
InChi Code
InChI=1S/C10H16N5O13P3S.4Na/c11-10-13-7-4(8(18)14-10)12-2-15(7)9-6(17)5(16)3(26-9)1-25-29(19,20)27-30(21,22)28-31(23,24)32;;;;/h2-3,5-6,9,16-17H,1H2,(H,19,20)(H,21,22)(H2,23,24,32)(H3,11,13,14,18);;;;/q;4*+1/p-4/t3-,5-,6-,9-;;;;/m1..../s1
InChi Key
HDHBWRGMEUXAOZ-ZVQJTLEUSA-J
Shipping & Storage Information
Storage
-80°C
Shipping
Dry ice in continental US; may vary elsewhere
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    2.7 mg

    $124

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    Product Description

    Guanosine 5'-[γ-thio]triphosphate (GTPγS) is a hydrolysis-resistant analog of GTP (Item No. 16060) that binds to and activates G proteins in the presence of magnesium.1 GTPγS has been used to obtain crystal structures of GTPases in their active states, as well as measure the stability of GTPases in the presence of ligands.2,3 A radiolabeled form of GTPγS, [35S]GTPγS, is commonly used in binding assays to determine agonism or antagonism at G protein-coupled receptors (GPCRs).1,4

    WARNING This product is not for human or veterinary use.

    References & Product Citations
    Product Description References

    1. Harrison, C., and Traynor, J.R. The [35S]GTPγS binding assay: Approaches and applications in pharmacology. Life Sci. 74(4), 489-508 (2003).

    2. Ihara, K., Muraguchi, S., Kato, M., et al. Crystal structure of human RhoA in a dominantly active form complexed with a GTP analogue. The Journal of Biological Chemisty 273(16), 9656-9666 (1998).

    3. Lito, P., Solomon, M., Li, L.-S., et al. Allele-specific inhibitors inactivate mutant KRAS G12C by a trapping mechanism. Science 351(6273), 604-608 (2016).

    4. Strange, P.G. Use of the GTPγS ([35S]GTPγS and Eu-GTPγS) binding assay for analysis of ligand potency and efficacy at G protein-coupled receptors. Br. J. Pharmacol. 161(6), 1238-1249 (2010).