A colorimetric β-glucuronidase substrate
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4-Nitrophenyl β-D-Glucuronide

Item No. 38756

Product Insert (PDF)Safety Data Sheet (SDS) (PDF)
Technical Information
Formal Name
4-nitrophenyl β-D-glucopyranosiduronic acid
CAS Number
10344-94-2
Molecular Formula
C12H13NO9
Formula Weight
315.2
Purity
≥98%
Formulation (Request formulation change)
A solid
Solubility(Learn about Variance in Solubility)
DMF: 20 mg/mlDMSO: 10 mg/mlEthanol: 10 mg/mlPBS (pH 7.2): 10 mg/ml
λmax
220, 296 nm
SMILES
O[C@H]([C@H]1O)[C@@H](O[C@@H]([C@H]1O)C(O)=O)OC(C=C2)=CC=C2[N+]([O-])=O
InChi Code
InChI=1S/C12H13NO9/c14-7-8(15)10(11(17)18)22-12(9(7)16)21-6-3-1-5(2-4-6)13(19)20/h1-4,7-10,12,14-16H,(H,17,18)/t7-,8-,9+,10-,12+/m0/s1
InChi Key
QSUILVWOWLUOEU-GOVZDWNOSA-N
Shipping & Storage Information
Storage
-20°C
Shipping
Room temperature in continental US; may vary elsewhere
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    50 mg

    $51

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    100 mg

    $72

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    250 mg

    $152

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    500 mg

    $253

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    Product Description

    4-Nitrophenyl β-D-glucuronide is a colorimetric substrate for β-glucuronidase.1 Upon enzymatic cleavage, p-nitrophenol is released, which can be quantified by colorimetric detection at 405 nm as a measure of β-glucuronidase activity.

    WARNING This product is not for human or veterinary use.

    References & Product Citations
    Product Description References

    1. Sadat Ebrahimi M.-M., Voss, Y., and Schönherr, H. Rapid detection of Escherichia coli via enzymatically triggered reactions in self-reporting chitosan hydrogels. ACS Appl. Mater. Interfaces 7(36), 20190-20199 (2015).