A fluorogenic substrate for carboxylesterase and AChE
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7-Acetoxy-4-methylcoumarin

Item No. 38909

Product Insert (PDF)Safety Data Sheet (SDS) (PDF)
Technical Information
Formal Name
7-(acetyloxy)-4-methyl-2H-1-benzopyran-2-one
CAS Number
2747-05-9
Synonyms
  • 4-Methylumbelliferyl Acetate
  • 4-MUA
  • NSC 1059
  • NSC 31658
  • NSC 44763
  • NSC 688806
Molecular Formula
C12H10O4
Formula Weight
218.2
Purity
≥95%
Formulation (Request formulation change)
A solid
Solubility(Learn about Variance in Solubility)
DMF: 20 mg/mlDMSO: 20 mg/ml
SMILES
O=C1OC2=C(C(C)=C1)C=CC(OC(C)=O)=C2
InChi Code
InChI=1S/C12H10O4/c1-7-5-12(14)16-11-6-9(15-8(2)13)3-4-10(7)11/h3-6H,1-2H3
InChi Key
HXVZGASCDAGAPS-UHFFFAOYSA-N
Shipping & Storage Information
Storage
-20°C
Shipping
Room temperature in continental US; may vary elsewhere
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    1 g

    $28

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    5 g

    $104

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    10 g

    $193

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    Product Description

    7-Acetoxy-4-methylcoumarin is a fluorogenic substrate for carboxylesterase and acetylcholinesterase (AChE).1,2 Upon hydrolysis of 7-acetoxy-4-methylcoumarin by carboxylic esterase or AChE, the fluorescent moiety 4-methylumbelliferyl (4-MU) is released, which displays pH-dependent excitation maxima of 330, 370, and 385 nm at pH 4.6, 7.4, and 10.4, respectively, and an emission maximum between 445-454 nm.3 7-Acetoxy-4-methylcoumarin has been used to detect the presence of carboxylesterases in isolated human lung tissue.1

    WARNING This product is not for human or veterinary use.

    References & Product Citations
    Product Description References

    1. Gabriele, M., Puccini, P., Lucchi, M., et al. Presence and inter-individual variability of carboxylesterases (CES1 and CES2) in human lung. Biochem. Pharmacol. 150, 64-71 (2018).

    2. Berman, H.A., and Leonard, K. Ligand exclusion on acetylcholinesterase. Biochemistry 29(47), 10640-10649 (1990).

    3. Zhi, H., Wang, J., Wang, S., et al. Fluorescent properties of hymecromone and fluorimetric analysis of hymecromone in compound dantong capsule. J. Spectrosc. 147128 (2013).