Cayman LipiDOT Strips^™ - PIPs Plus

Cayman LipiDOT Strips^™ - PIPs Plus is a nitrocellulose membrane-based screening tool for the identification of protein-lipid interactions and assessment of a protein's relative lipid-binding specificity and affinity. It contains several types of lipids, such as phospholipids, including a variety of phosphatidylinositol phosphate (PIP) species, lysophospholipids, and cardiolipin, as well as cholesterol and sphingolipids. These lipids interact with a variety of proteins to regulate signaling events, nutrient and energy metabolism, lipid transport, and cell adhesion, among other processes.¹

Cayman LipiDOT Strips^™ - Plus contains 22 biologically relevant lipids and two blank (blue) spots for adding a positive control (protein) or using as a negative control and/or additional lipids in the protein-lipid overlay (PLO) assay. The lipids and controls are spotted on a 3.175 cm x 6 cm supported nitrocellulose membrane in a 3 x 8 grid. This product can be used with Cayman's LipiDOT™ Strips Developing Chamber (Item No. 39380) for the blocking, incubation, and washing steps.

WARNING This product is not for human or veterinary use.

1. Corradi, V., Sejdiu, B.I., Mesa-Galloso, H., et al. Emerging diversity in lipid-protein interactions. Chem. Rev. 119(9), 5775-5848 (2019).