Nitrotyrosine Affinity Sorbent

Nitrotyrosine is a post-translational modification that is formed by the nitration of tyrosine.¹ It exists in a free or protein-bound form and is commonly used as a marker of nitrosative or oxidative stress.² Nitrotyrosine residues have been found in a variety of proteins, including LDL, surfactant protein A, angiotensin II, and human and bovine serum albumin.³ Nitrotyrosine levels are increased in the affected tissues of numerous pathological conditions, including atherosclerosis, cancer, ulcerative colitis, Alzheimer's disease, and Parkinson's disease.³ Autoantibodies that recognize nitrotyrosinated proteins are increased in the synovium of patients with rheumatoid arthritis and are positively correlated with joint and tendon inflammation.¹ Cayman's Nitrotyrosine Affinity Sorbent is designed for immunoprecipitation (IP) of nitrated proteins from biological samples. The nitrotyrosine affinity sorbent consists of Cayman's Nitrotyrosine Monoclonal Antibody (Item No. 189542) coupled to protein A agarose. This is an effective way to concentrate nitrated proteins for subsequent detection by a different anti-nitrotyrosine antibody by Western blot (WB).

WARNING This product is not for human or veterinary use.

1. Smallwood, M.J., Nissim, A., Knight, A.R., et al. Oxidative stress in autoimmune rheumatic diseases. Free Radic. Biol. Med. 125, 3-14 (2018).

2. Teixeira, D., Fernandes, R., Prudêncio, C., et al. 3-Nitrotyrosine quantification methods: Current concepts and future challenges. Biochimie 125, 1-11 (2016).

3. Oldreive, C., and Rice-Evans, C. The mechanisms for nitration and nitrotyrosine formation in vitro and in vivo: Impact of diet. Free Radic. Res. 35(3), 215-231 (2001).