A fluorescent dye and DNA intercalator
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Ethidium (bromide)

Item No. 39132

Product Insert (PDF)Safety Data Sheet (SDS) (PDF)
Technical Information
Formal Name
3,8-diamino-5-ethyl-6-phenyl-phenanthridinium, monobromide
CAS Number
1239-45-8
Synonyms
  • Dromilac
  • Homidium Bromide
Molecular Formula
C21H20N3 • Br
Formula Weight
394.3
Purity
≥95%
Emission
595 nm
Excitation
525 nm
Formulation (Request formulation change)
A solid
Solubility(Learn about Variance in Solubility)
DMSO: 10 mg/ml
SMILES
CC[N+]1=C(C=C2N)C(C=C2)=C(C=C3)C(C=C3N)=C1C4=CC=CC=C4.[Br-]
InChi Code
InChI=1S/C21H19N3.BrH/c1-2-24-20-13-16(23)9-11-18(20)17-10-8-15(22)12-19(17)21(24)14-6-4-3-5-7-14;/h3-13,23H,2,22H2,1H3;1H
InChi Key
ZMMJGEGLRURXTF-UHFFFAOYSA-N
Shipping & Storage Information
Storage
-20°C
Shipping
Room temperature in continental US; may vary elsewhere
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    Product Description

    Ethidium is a phenanthridine fluorescent dye and DNA intercalator.1,2 It displays an emission at 595 nm upon excitation at 525 nm.1 Ethidium inhibits the DNA-unwinding and ATPase activities of P. falciparum DNA helicase (IC50s = 1.5 and 2.5 µM, respectively) and is active against P. falciparum in vitro.3 It completely inhibits E. coli RNA polymerase when used at a concentration of 100 µmol/ml and is active against Mycoplasma when used at concentrations ranging from 0.5 to 8 µg/ml.4,5 Ethidium (8 mg/kg) increases lifespan in 6C3HED-OG and L5178Y murine lymphoma models.6 It has commonly been used to stain DNA in agarose gel electrophoresis and in displacement assays to determine compound binding to DNA.7,8

    WARNING This product is not for human or veterinary use.