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The NaveniFlex™ Tissue Atto647N kit is an in situ proximity ligation assay kit optimized for tissue samples. The kit enables fluorescence visualization of protein-protein interactions or post-translational modifications such as phosphorylation and ensures high specificity when localizing single proteins. The kit should be used with the user’s primary antibodies of choice (raised in mouse and rabbit).
Validated on FFPE and Fresh frozen human and mouse tissue sections. Validated on several types of tissues, both healthy and cancerous, for example, colon, tonsil, ovary, kidney, breast, spleen, brain, and skin.
Compatible with traditional immunostaining equipment, the kit uses secondary antibody detection to produce an amplified signal in the form of a fluorescent dot, thus facilitating the quantification of the readout.
The kit is based on our Naveni™ Proximity Ligation Technology, with TriFlex Navenibodies conjugated to proprietary oligo arms. The technology ensures specific and sensitive detection; learn more about Naveni technology.
Included in the kit:
• Anti-mouse Navenibody
• Anti-rabbit Navenibody
• Detection fluorophore; Atto647N
• Buffers for blocking and dilutions, and all detection reagents
• The Navenibody solution has a working volume of 4000 µl
Mouse and rabbit primary antibodies are not included in the kit. For guidance on selecting a primary antibody for the protein of interest, please contact Cayman's Technical Support team.
WARNING This product is not for human or veterinary use.