For immunochemical detection of citrullinated collagen type II
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Citrullinated Collagen Type II Chimeric Rabbit Monoclonal Antibody (Clone ACC1)

Item No. 39297

Product Insert (PDF)Safety Data Sheet (SDS) (PDF)
Technical Information
Synonyms
  • Alpha-1 Type II Collagen
  • Collagen Alpha-1(II) Chain
Immunogen
Bovine collagen type IIA
Clone Designation
ACC1
Formulation (Request formulation change)
200 µg of protein A-affinity purified monoclonal antibody
Storage Buffer
PBS with 0.02% Proclin™ 300
Host
Chimeric monoclonal antibody
Isotype
IgG
Applications
ELISA, IHC
Cross Reactivity
(+) Citrullinated form of the triple helical collagen type II C1 epitope (amino acids 359-369)(+) Denatured collagen type II(+) Denatured collagen type II triple helical C1 epitope (amino acids 359-369)
Species Reactivity
(+) Human(+) Mouse(+) Bovine
UniProt Accession №
P02458
Shipping & Storage Information
Storage
-20°C
Shipping
Wet ice in continental US; may vary elsewhere
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    200 µg

    $473

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    Product Description

    Collagen type II is a fibril-forming heterodimeric protein present in cartilage, cartilaginous zones of tendons, and intervertebral discs that is subject to citrullination by peptidyl arginine deiminase 2 (PAD2) and PAD4.1,2 Citrullinated collagen type II and autoantibodies to citrullinated collagen type II have been found in synovial fluid isolated from patients with rheumatoid arthritis, with autoantibodies appearing years prior to clinical disease onset.2,3,4 Anti-citrullinated collagen type II antibodies induce proteoglycan depletion of cartilage and arthritis in mice.4,5 Cayman’s Citrullinated Collagen Type II Chimeric Rabbit Monoclonal Antibody (Clone ACC1) was produced recombinantly from the sequence of the original anti-citrullinated collagen type II monoclonal antibody, ACC1, substituting the mouse IgG2a variable domain for the rabbit IgG variable domain, and can be used for ELISA and immunohistochemistry (IHC) applications. The ACC1 antibody was generated by immunizing Cia5 congenic B10.RIII mice with bovine collagen type II.5

    WARNING This product is not for human or veterinary use.

    References & Product Citations
    Product Description References

    1. Sorushanova, A., Delgado, L.M., Wu, Z., et al. The collagen suprafamily: From biosynthesis to advanced biomaterial development. Adv. Mater. 31(1), e1801651 (2019).

    2. Foulquier, C., Sebbag, M., Clavel, C., et al. Peptidyl arginine deiminase type 2 (PAD-2) and PAD-4 but not PAD-1, PAD-3, and PAD-6 are expressed in rheumatoid arthritis synovium in close association with tissue inflammation. Arthritis Rheum. 56(11), 3541-3553 (2007).

    3. Uysal, H., Nandakumar, K.S., Kessel, C., et al. Antibodies to citrullinated proteins: Molecular interactions and arthritogenicity. Immunol. Rev. 233(1), 9-33 (2009).

    4. Ge, C., Tong, D., Liang, B., et al. Anti-citrullinated protein antibodies cause arthritis by cross-reactivity to joint cartilage. JCI Insight 2(13), e93688 (2017).

    5. Uysal, H., Bockermann, R., Nandakumar, K.S., et al. Structure and pathogenicity of antibodies specific for citrullinated collagen type II in experimental arthritis. J. Exp. Med. 206(2), 449-462 (2009).