For immunochemical detection of influenza A H1N1 (Swine Flu 2009) HA
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Influenza A H1N1 (Swine Flu 2009) HA Monoclonal Antibody (Clone 9G1G8)

Item No. 39541

Product Insert (PDF)
Technical Information
Synonyms
  • Influenza A H1N1 (Swine Flu 2009) Hemagglutinin
Immunogen
Recombinant influenza A H1N1 (Swine Flu 2009) HA
Clone Designation
9G1G8
Formulation (Request formulation change)
20 or 100 µl of protein A-affinity purified monoclonal antibody
Storage Buffer
0.2 μm filtered solution in PBS
Host
Mouse
Isotype
IgG2b
Applications
ELISA, WB
Cross Reactivity
(+) H1N1 (A/California/04/2009) HA(+) H1N1 (A/California/07/2009) HA(+) H1N2 (A/swine/Guangxi/13/2006) HA(+) H1N3 (A/duck/NZL/160/1976) HA(+) H5N1 (A/Anhui/1/2005) HA(+) H5N1 (A/Vietnam/1194/2004) HA(+) H5N1 (A/Indonesia/5/2005) HA(+) H5N1 (A/turkey/Turkey/1/2005) HA(+) H5N1 (A/bar-headed goose/Qinghai/14/2008) HA(-) H1N1 (A/Brisbane/59/2007) HA(-) H1N1 (A/BrevigMission/1/1918) HA(-) H1N1 (A/Solomon Islands/3/2006) HA(-) H1N1 (A/Ohio/UR06-0091/2007) HA(-) H1N1 (A/New Caledonia/20/1999) HA(-) H1N1 (A/Puerto Rico/8/1934) HA(-) H1N1 (A/WSN/1933) HA(-) H3N2 (A/Brisbane/10/2007) HA(-) Influenza B (B/Florida/4/2006) HA
Shipping & Storage Information
Storage
4°C
Shipping
Room temperature in continental US; may vary elsewhere
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Certificates of Analysis & Batch Specific Data

Provide batch numbers separated by commas to download or request available product inserts, QC sheets, certificates of analysis, data packs, and GC-MS data.

    20 µl

    $99

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    100 µl

    $323

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    Cayman Chemical
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    Product Description

    Influenza A H1N1 HA is a type I membrane glycoprotein involved in receptor binding and virus-host cell fusion.1,2 It is produced as a precursor protein, HA0, which is composed of a stalk and head domain and forms homotrimers on the viral surface.3,1 The HA0 precursor is cleaved into subunits, HA1 and HA2, which are responsible for host cell surface receptor binding and endosomal membrane fusion, respectively, and this cleavage is required for endosomal fusion.1 For influenza A and influenza B, which are low pathogenic influenza viruses, cleavage occurs via trypsin-like proteases, such as transmembrane serine protease 2 (TMRPSS2), which is essential for influenza A HA, but not influenza B HA, cleavage.4,5,6 Cleaved influenza A H1N1 HA binds to terminal α2,6- or α2,3-sialic acids on glycoproteins or glycolipids on the host cell surface via the receptor-binding domain in the HA1 subunit, which triggers endocytosis of the virus and trafficking of the vesicle into the endosome.3,7,8 The low pH environment of the endosome triggers viral rearrangement into a prefusion conformation, and the HA2 subunit facilitates fusion with the endosomal membrane to release viral ribonucleoproteins into the cytosol where they are relocated to the nucleus for viral replication.3 Cayman’s Influenza A H1N1 (Swine Flu 2009) HA Monoclonal Antibody (Clone 9G1G8) can be used for ELISA and Western blot (WB) applications.

    WARNING This product is not for human or veterinary use.

    References & Product Citations
    Product Description References

    1. Russell, C.J. Hemagglutinin stability and its impact on influenza A virus infectivity, pathogenicity, and transmissibility in avians, mice, swine, seals, ferrets, and humans. Viruses 13(5), 746 (2021).

    2. Gamblin, S.J., and Skehel, J.J. Influenza hemagglutinin and neuraminidase membrane glycoproteins. The Journal of Biological Chemisty 285(37), 28403-28409 (2010).

    3. Dou, D., Revol, R., Östbye, H., et al. Influenza A virus cell entry, replication, virion assembly and movement. Front. Immunol. 9, 1581 (2018).

    4. Sakai, K., Ami, Y., Nakajima, N., et al. TMPRSS2 independency for haemagglutinin cleavage in vivo differentiates influenza B virus from influenza A virus. Sci. Rep. 6, 29430 (2016).

    5. Böttcher-Friebertshäuser, E., Lu, Y., Meyer, D., et al. Hemagglutinin activating host cell proteases provide promising drug targets for the treatment of influenza A and B virus infections. Vaccine 30(51), 7374-7380 (2012).

    6. Limburg, H., Harbig, A., Bestle, D., et al. TMPRSS2 is the major activating protease of influenza A virus in primary human airway cells and influenza B virus in human type II pneumocytes. J. Virol. 93(21), e00649-00619 (2019).

    7. Long, J.S., Mistry, B., Haslam, S.M., et al. Host and viral determinants of influenza A virus species specificity. Nat. Rev. Microbiol. 17(2), 67-81 (2019).

    8. Wang, Q., Tian, X., Chen, X., et al. Structural basis for receptor specificity of influenza B virus hemagglutinin. Proc. Natl. Acad. Sci. USA 104(43), 16874-16879 (2007).