A chloride channel inhibitor
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NS 3728

Item No. 39988

Product Insert (PDF)Safety Data Sheet (SDS) (PDF)
Technical Information
Formal Name
N-[3,5-bis(trifluoromethyl)phenyl]-N′-[4-bromo-2-(2H-tetrazol-5-yl)phenyl]-urea
CAS Number
265646-85-3
Synonyms
  • Endovion
  • SCO 101
Molecular Formula
C16H9BrF6N6O
Formula Weight
495.2
Purity
≥95%
Formulation (Request formulation change)
A solid
Solubility(Learn about Variance in Solubility)
DMSO: Soluble: ≥10 mg/mlEthanol: Sparingly soluble: 1-10 mg/ml
SMILES
O=C(NC1=CC(C(F)(F)F)=CC(C(F)(F)F)=C1)NC(C=C2)=C(C3=NN=NN3)C=C2Br
InChi Code
InChI=1S/C16H9BrF6N6O/c17-9-1-2-12(11(6-9)13-26-28-29-27-13)25-14(30)24-10-4-7(15(18,19)20)3-8(5-10)16(21,22)23/h1-6H,(H2,24,25,30)(H,26,27,28,29)
InChi Key
AEFYFGMSRKDXHZ-UHFFFAOYSA-N
Shipping & Storage Information
Storage
-20°C
Shipping
Room temperature in continental US; may vary elsewhere
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    5 mg

    $109

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    10 mg

    $196

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    25 mg

    $355

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    50 mg

    $491

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    Product Description

    NS 3728 is a chloride channel inhibitor.1,2 It inhibits the volume-regulated anion channel (VRAC) in HEK293 cells and chloride conductance in washed isolated human erythrocytes (IC50s = 0.4 and 0.6 µM, respectively).1 NS 3728 also inhibits calcium-activated chloride currents in Ehrlich-Lettre ascites carcinoma cells (IC50s = 2.11 and 0.7 µM at +95 and -50 mV, respectively).2 It reduces the proliferation of Ehrlich-Lettre ascites cells and inhibits IFN-γ-induced nitric oxide (NO) production in BV-2 microglia (IC50s = 0.52 and 3.7 µM, respectively).2,3

    WARNING This product is not for human or veterinary use.

    References & Product Citations
    Product Description References

    1. Hélix, D., Strøbaek, B.H.D., and Christophersen, P. Inhibition of the endogenous volume-regulated anion channel (VRAC) in HEK293 cells by acidic di-aryl-ureas. J. Membr. Biol. 196(2), 83-94 (2003).

    2. Klausen, T.K., Bergdahl, A., Hougaard, C., et al. Cell cycle-dependent activity of the volume- and Ca2+-activated anion currents in Ehrlich Lettre ascites cells. J. Cell. Physiol. 210(3), 831-842 (2007).

    3. Kjaer, K., Strøbaek, D., Christophersen, P., et al. Chloride channel blockers inhibit iNOS expression and NO production in IFNγ-stimulated microglial BV2 cells. Brain Res. 1281, 15-24 (2009).