Solid phase extraction cartridges used for purification
Features
  • Solid phase extraction cartridges containing immobilized C18
  • For reverse-phase extraction of non-polar to moderately polar compounds
  • Commonly used for the partial purification of eicosanoids
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SPE Cartridges (C18) (1 ml)

Item No. 400077

Technical Information
Synonyms
  • C18 Column
  • Solid Phase Extraction Cartridges
Formulation
1 ml column containing 100 mg sorbent
Shipping & Storage Information
Storage
Room temperature
Shipping
Room temperature in continental US; may vary elsewhere
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Certificates of Analysis & Batch Specific Data

Provide batch numbers separated by commas to download or request available product inserts, QC sheets, certificates of analysis, data packs, and GC-MS data.

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    Product Description

    This product may be used for purification of various lipid mediators such as prostaglandins and hydroxylated fatty acids from various sample types prior to analysis by LC-MS/MS or ELISA. References are provided below for applicable C18 sample purification methods prior to LC-MS analysis.1,2,3,4 For sample purification prior to analysis by ELISA, we recommend consulting the appropriate assay kit booklet for instructions.

    WARNING This product is not for human or veterinary use.

    References & Product Citations
    Product Description References

    1. Maddipati, K.R., and Zhou, S.L. Stability and analysis of eicosanoids and docosanoids in tissure culture media. Prostaglandins Other Lipid Mediat. 94(1-2), 59-72 (2011).

    2. Maddipati, K.R., Romero, R., Chaiworapongsa, T., et alEicosanomic profiling reveals dominance of the epoxygenase pathway in human amniotic fluid at term in spontaneous labor. The FASEB Journal 28(11), 4835-4846 (2014).

    3. Powell, W.S. Rapid extraction of arachidonic acid metabolites from biological samples using octadecylsilyl silica. Methods in Enzymology 86, 467-477 (1982).

    4. Song, W.L., Wang, M., Ricciotti, E., et alTetranor PGDM, an abundant urinary metabolite reflects biosynthesis of prostaglandin D2 in mice and humans. The Journal of Biological Chemisty 283(2), 1179-1188 (2008).