An MC3-based LNP solution for expressing luciferase
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LipidLaunch™ MC3 LNP (Luciferase)

Item No. 40108

Product Insert (PDF)Safety Data Sheet (SDS) (PDF)
Technical Information
Synonyms
  • LipidLaunch™ DLin-MC3-DMA LNP (Luciferase)
Formulation (Request formulation change)
TBS, pH 7.5, with 10% sucrose
Shipping & Storage Information
Storage
-80°C
Shipping
Dry ice in continental US; may vary elsewhere
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Certificates of Analysis & Batch Specific Data

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    5 µg

    $395

    Add

    50 µg

    $1,383

    Add
    Cayman Chemical
    Product Description

    LipidLaunch™ MC3 LNP (Luciferase) is a solution containing lipid nanoparticles (LNPs) composed of the ionizable cationic amino lipid DLin-MC3-DMA (MC3; Item No. 34364), cholesterol (Item No. 9003100), 1,2-distearoyl-sn-glycero-3-PC (1,2-DSPC; Item No. 15100), and the lipid excipient DMG-PEG(2000) (Item No. 33945) at a molar ratio of 50:38.5:10:1.5 and encapsulating mRNA encoding firefly luciferase protein. It is intended for proof-of-concept experiments to determine whether MC3-based LNPs can effectively lead to the expression of a protein of interest in a target cell type, either in vitro or in vivo. Firefly luciferase is an enzyme that catalyzes the oxidation of ATP-dependent D-luciferin, resulting in chemiluminescence at a wavelength of approximately 560 nm.

    Suggested in vitro use: Thaw LNPs on ice with occasional gentle swirling (do not vortex). Using a gentle pipetting technique, dilute 1:100-1:500 in complete cell culture media (with serum) and add to subconfluent cells in a luminescence-compatible tissue culture plate. Use a luciferase reporter assay substrate and read luminescence on a plate reader. Optimal conditions are highly dependent on cell type.

    WARNING This product is not for human or veterinary use.