Active • Host: E. coli • AA: 1-337 • Tag: C-terminal His • MW: 37.5 kDa
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Malate Dehydrogenase 1 (human, recombinant)

Item No. 40110

Product Insert (PDF)Safety Data Sheet (SDS) (PDF)
Technical Information
Synonyms
  • Aromatic α-keto Acid Reductase
  • Cytosolic Malate Dehydrogenase
  • MDH1
  • Mor2
  • Soluble Malate Dehydrogenase
Purity
≥85% estimated by SDS-PAGE
Source
Active recombinant human C-terminal His-tagged MDH1 expressed in E. coli
Amino Acids
1-337
MW
37.49 kDa
Formulation (Request formulation change)
25 mM HEPES, pH 7.5, with 150 mM sodium chloride, 5% glycerol, and 1 mM mercaptoethanol
Host
E. coli
UniProt Accession №
P40925
Shipping & Storage Information
Storage
-80°C
Shipping
Dry ice in continental US; may vary elsewhere
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    100 µg

    $534

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    Cayman Chemical
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    Product Description

    Malate dehydrogenase 1 (MDH1) is an NAD-dependent dehydrogenase and oxidoreductase.1 It is composed of an N-terminal NAD binding domain and a C-terminal substrate binding domain that reversibly catalyzes the conversion of malate to oxaloacetate in the cytosol.1,2 MDH1 functions as a homodimer and is ubiquitously expressed in the human body, functioning as a key metabolic enzyme in glycolysis and the urea acid cycle.1 MDH1 supports increased rates of cancer glycolysis by serving as both a carbon and NAD supplier in lieu of, and in addition to, lactate dehydrogenase (LDH).3 MDH1 expression is amplified in a variety of cancers, including lung squamous cell carcinoma, diffuse B cell lymphoma, as well as bladder and pancreatic cancers, and its higher expression is negatively correlated with patient survival. Oncogenic K-Ras-induced glycosylation of MDH1, but not the mitochondrial MDH2 isoform, stabilizes the substrate-binding domain, increases MDH1 activity, and increases proliferation in pancreatic cancer cells.4 MDH1 activity is increased in brain homogenates from patients with early Alzheimer’s disease compared to brain homogenates from age-matched individuals without Alzheimer's disease.5 Cayman’s Malate Dehydrogenase 1 (human, recombinant) protein can be used for ELISA, enzyme assay, and Western blot (WB) applications.

    WARNING This product is not for human or veterinary use.

    References & Product Citations
    Product Description References

    1. McCue, W.M., and Finzel, B.C. Structural characterization of the human cytosolic malate dehydrogenase I. ACS Omega 7(1), 207-214 (2021).

    2. Minárik, P., Tomásková, N., Kollárová, M., et al. Malate dehydrogenases — structure and function. Gen. Physiol. Biophys. 21(3), 257-265 (2002).

    3. Hanse, E.A., Ruan, C., Kachmann, M., et al. Cytosolic malate dehydrogenase activity helps support glycolysis in actively proliferating cells and cancer. Oncogene 36(27), 3915-3924 (2017).

    4. Zhu, Q., Zhou, H., Wu, L., et al. O-GlcNAcylation promotes pancreatic tumor growth by regulating malate dehydrogenase 1. Nat. Chem. Biol. 18(10), 1087-1095 (2022).

    5. Reed, T.T., Pierce, W.M., Markesbery, W.R., et al. Proteomic identification of HNE-bound proteins in early Alzheimer disease: Insights into the role of lipid peroxidation in the progression of AD. Brain Res. 1274, 66-76 (2009).