Active • Host: E. coli • AA: 42-711 • Tag: N-terminal His • MW: 76.5 kDa
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ACSL4 (42-711) (human, recombinant)

Item No. 40327

Product Insert (PDF)Safety Data Sheet (SDS) (PDF)
Technical Information
Synonyms
  • ACS4
  • FACL4
  • Fatty-Acid-Coenzyme A Ligase 4
  • Long-chain Acyl-CoA Synthetase 4
  • Long-chain Fatty Acid-CoA Ligase 4
Purity
≥70% estimated by SDS-PAGE
Source
Active recombinant human N-terminal His-tagged ACSL4 expressed in E. coli
Amino Acids
42-711
MW
76.5 kDa
Formulation (Request formulation change)
50 mM Tris, pH 8.0, with 150 mM sodium chloride, 10% glycerol, and 5 mM magnesium chloride
Host
E. coli
UniProt Accession №
O60488
Shipping & Storage Information
Storage
-80°C
Shipping
Dry ice in continental US; may vary elsewhere
Certificates of Analysis & Batch Specific Data

Provide batch numbers separated by commas to download or request available product inserts, QC sheets, certificates of analysis, data packs, and GC-MS data.

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    20 µg

    $428

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    Product Description

    Long-chain acyl-CoA synthetase 4 (ACSL4) is a CoA ligase and member of the ACSL family.1,2 It is composed of an N-terminal membrane-binding domain, a transmembrane domain, an adenosine adaptor region, and a C-terminal catalytic domain.2 ACSL4 is primarily found in the mitochondria, peroxisomes, endoplasmic reticulum (ER), and plasma membrane.1,3 It is generally involved in fatty acid degradation via β-oxidation and lipid synthesis.2 Specifically, ACSL4 ligates 20-chain PUFAs, such as eicosapentaenoic acid (Item Nos. 90110 | 90110.1 | 21908) or arachidonic acid (Item Nos. 90010 | 90010.1 | 10006607), to CoA to form fatty acyl-CoA esters, which are then used for lipid synthesis, plasma membrane repair, and as a carbon source in the citric acid cycle, among other processes.2,4 However, this process can be dysregulated by increased intracellular levels of ferrous iron (Fe2+) and reactive oxygen species (ROS) that further oxidize these PUFA fatty acyl-CoA esters into lipid peroxides that contribute to the development of ferroptosis.3,5,6 Increased expression of ACSL4 is associated with higher cancer staging in patients with prostate cancer.7 Cayman's ACSL4 (42-711) (human, recombinant) protein can be used for enzyme activity, ELISA, and Western blot (WB) applications.

    WARNING This product is not for human or veterinary use.

    References & Product Citations
    Product Description References

    1. Quan, J., Bode, A.M., and Luo, X. ACSL family: The regulatory mechanisms and therapeutic implications in cancer. Eur. J. Pharmacol. 909, 174397 (2021).

    2. Chen, F., Kang, R., Liu, J., et al. The ACSL4 network regulates cell death and autophagy in diseases. Biology (Basel) 12(6), 864 (2023).

    3. Yang, Y., Zhu, T., Wang, X., et al. ACSL3 and ACSL4, distinct roles in ferroptosis and cancers. Cancers (Basel) 14(23), 5896 (2022).

    4. Trub, A.G., and Hirschey, M.D. Reactive acyl-CoA species modify proteins and induce carbon stress. Trends Biochem. Sci. 43(5), 369-379 (2018).

    5. Liao, P., Wang, W., Wang, W., et al. CD8+ T cells and fatty acids orchestrate tumor ferroptosis and immunity via ACSL4. Cancer Cell 40(4), 365-378 (2022).

    6. Zhang, H.-L., Hu, B.-X., Li, Z.-L., et al. PKCβII phosphorylates ACSL4 to amplify lipid peroxidation to induce ferroptosis. Nat. Cell Biol. 24(1), 88-98 (2022).

    7. Wu, X., Deng, F., Li, Y., et al. ACSL4 promotes prostate cancer growth, invasion and hormonal resistance. Oncotarget 6(42), 44849-44863 (2015).