Host: Insect cells • AA: 1-413 (full length) • Tag: C-terminal His • MW: 46.51 kDa
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MERS-CoV Nucleocapsid Protein (recombinant)

Item No. 40878

Product Insert (PDF)Safety Data Sheet (SDS) (PDF)
Technical Information
Synonyms
  • MERS-CoV Nucleoprotein
  • MERS-CoV N Protein
Purity
≥90% as estimated by SDS-PAGE
Endotoxin Testing
<1.0 EU/µg, determined by the LAL endotoxin assay
Source
Recombinant MERS-CoV C-terminal His-tagged nucleocapsid expressed in insect cells
Amino Acids
1-413 (full length)
MW
46.51 kDa
Formulation (Request formulation change)
Lyophilized from sterile 20 mM Tris, pH 8.0, with 500 mM sodium chloride and 10% glycerol
Host
Insect cells
UniProt Accession №
K0BVN3
Shipping & Storage Information
Storage
-80°C
Shipping
Dry ice in continental US; may vary elsewhere
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    100 µg

    $493

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    Cayman Chemical
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    Product Description

    Middle East respiratory syndrome coronavirus (MERS-CoV) is an enveloped positive-stranded RNA virus, a member of the Betacoronavirus genus, and a causative agent of MERS, an acute respiratory disease that often leads to pneumonia and renal failure.1,2 The MERS-CoV nucleocapsid protein is composed of an intrinsically disordered region (IDR), an N-terminal domain that binds RNA, a flexible linker containing serine and arginine phosphorylation sites, a C-terminal domain that also participates in RNA binding and is responsible for dimerization, and another IDR.3,4 Coronavirus nucleocapsid proteins, including the MERS-CoV nucleocapsid protein, package the viral genome into a ribonucleoprotein (RNP) complex.4 In addition, the MERS-CoV nucleocapsid protein interacts with the ubiquitin ligase TRIM25 and inhibits the activation of retinoic acid-inducible gene I (RIG-I) and phosphorylation of IFN regulatory factor 3 (IRF3) and NF-κB.5 It reduces IFN-β and IFN-λ1 promoter activity induced by the RIG-I caspase activation and recruitment domain (RIG-I-CARD), but not MDA5-CARD, and reduces Sendai virus-induced increases in IFN-β and IFN-λ1 mRNA levels. Cayman's MERS-CoV Nucleocapsid Protein (recombinant) consists of 424 amino acids and has a calculated molecular weight of 46.51 kDa.

    WARNING This product is not for human or veterinary use.

    References & Product Citations
    Product Description References

    1. Du, L., Yang, Y., Zhou, Y., et al. MERS-CoV spike protein: A key target for antivirals. Expert Opin. Ther. Targets 21(2), 131-143 (2017).

    2. Rabaan, A.A., Al-Ahmed, S.H., Haque, S., et al. SARS-CoV-2, SARS-CoV, and MERS-CoV: A comparative overview. Infez. Med. 28(2), 174-184 (2020).

    3. Papageorgiou, N., Lichière, J., Baklouti, A., et al. Structural characterization of the N-terminal part of the MERS-CoV nucleocapsid by X-ray diffraction and small-angle X-ray scattering. Acta Crystallogr. D. Struct. Biol. 72(Pt 2), 192-202 (2016).

    4. Zhang, B., Tian, J., Zhang, Q., et al. Comparing the nucleocapsid proteins of human coronaviruses: Structure, immunoregulation, vaccine, and targeted drug. Front. Mol. Biosci. 9, 761173 (2022).

    5. Chang, C.-Y., Liu, H.M., Chang, M.-F., et al. Middle East respiratory syndrome coronavirus nucleocapsid protein suppresses type I and type III interferon induction by targeting RIG-I signaling. J. Virol. 94(13), e00099-00020 (2020).