Host: E. coli • AA: 13-128 • Tag: N-Terminal His
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SARS-CoV-2 nsp1 (recombinant)

Item No. 40879

Product Insert (PDF)
Technical Information
Synonyms
  • SARS-CoV-2 Host Translation Inhibitor nsp1
  • SARS-CoV-2 Non-structural Protein 1
  • Severe Acute Respiratory Syndrome Coronavirus 2 nsp1
Purity
≥90%
Endotoxin Testing
<1.0 EU/μg, determined by the LAL endotoxin assay
Source
Recombinant SARS-CoV-2 N-terminal His-tagged nsp1 expressed in E. coli
Amino Acids
13-128
Formulation (Request formulation change)
50 mM Tris-HCl, pH 7.5, 200 mM sodium chloride, and 20% glycerol
UniProt Accession №
P0DTD1
Shipping & Storage Information
Storage
-80°C
Shipping
Dry ice in continental US; may vary elsewhere
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Certificates of Analysis & Batch Specific Data

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    100 µg

    $601

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    500 µg

    $1,947

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    Product Description

    Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is an enveloped positive-stranded RNA virus and the causative agent of COVID-19, a primarily respiratory illness characterized by fever, cough, and shortness of breath that can lead to life-threatening complications.1,2,3,4,5 The SARS-CoV-2 genome contains approximately 30 kilobases and 14 open reading frames (ORFs) that encode four structural proteins: spike, envelope, membrane, and nucleocapsid, as well as 16 non-structural proteins and 9 accessory factors.6 SARS-CoV-2 non-structural protein 1 (nsp1) is a multifunctional protein and virulence factor that inhibits host mRNA translation, including translation of antiviral proteins such as IFN-β and IFN-λ1.7,8,9 It is composed of an N-terminal domain and a C-terminal domain connected via an unstructured 20-amino acid residue linker.10,9 The SARS-CoV-2 nsp1 C-terminal domain binds to the mRNA entry site of the 40S subunit of the host ribosome, which prevents host mRNA from entering, while the N-terminal binds to the 40S decoding center preventing accurate alignment of tRNA and mRNA.8,10,9 SARS-CoV-2 nsp1 also induces host mRNA degradation, prevents mRNA export from the nucleus to the cytoplasm, and binds to pre-rRNA in the nucleolus to disrupt rRNA biogenesis.11,9,12,7

    WARNING This product is not for human or veterinary use.

    References & Product Citations
    Product Description References

    1. Kandeel, M., Ibrahim, A., Fayez, M., et al. From SARS and MERS CoVs to SARS-CoV-2: Moving toward more biased codon usage in viral structural and nonstructural genes. J. Med. Virol. 92(6), 660-666 (2020).

    2. Lu, R., Zhao, X., Li, J., et al. Genomic characterisation and epidemiology of 2019 novel coronavirus: Implications for virus origins and receptor binding. Lancet 395(10224), 565-574 (2020).

    3. Meo, S.A., Alhowikan, A.M., Al-Khlaiwi, T., et al. Novel coronavirus 2019-nCoV: Prevalence, biological and clinical characteristics comparison with SARS-CoV and MERS-CoV. Eur. Rev. Med. Pharmacol. Sci. 24(4), 2012-2019 (2020).

    4. Klok, F.A., Kruip, M.J.H.A., van der Meer, N.J.M., et al. Incidence of thrombotic complications in critically ill ICU patients with COVID-19. Thromb. Res. 191, 145-147 (2020).

    5. Yang, F., Shi, S., Zhu, J., et al. Analysis of 92 deceased patients with COVID-19. J. Med. Virol. 92(11), 2511-2515 (2020).

    6. Romano, M., Ruggiero, A., Squeglia, F., et al. A structural view of SARS-CoV-2 RNA replication machinery: RNA synthesis, proofreading and final capping. Cells 9(5), 1267 (2020).

    7. Yerlici, V.T., Astori, A., Kejiou, N.S., et al. SARS-CoV-2 targets ribosomal RNA biogenesis. Cell Rep. 43(3), 113891 (2024).

    8. Thoms, M., Buschauer, R., Ameismeier, M., et al. Structural basis for translational shutdown and immune evasion by the Nsp1 protein of SARS-CoV-2. Science 369(6508), 1249-1255 (2020).

    9. Karousis, E.D. The art of hijacking: How Nsp1 impacts host gene expression during coronaviral infections. Biochem. Soc. Trans. 52(1), 481-490 (2024).

    10. Schubert, K., Karousis, E.D., Jomaa, A., et al. SARS-CoV-2 Nsp1 binds the ribosomal mRNA channel to inhibit translation. Nat. Struct. Mol. Biol. 27(10), 959-966 (2020).

    11. Mendez, A.S., Ly, M., González-Sánchez, A.M., et al. The N-terminal domain of SARS-CoV-2 nsp1 plays key roles in suppression of cellular gene expression and preservation of viral gene expression. Cell Rep. 37(3), 109841 (2021).

    12. Zhang, K., Miorin, L., Makio, T., et al. Nsp1 protein of SARS-CoV-2 disrupts the mRNA export machinery to inhibit host gene expression. Sci. Adv. 7(6), eabe7386 (2021).