Active • Host: E. coli • AA: 1,480-1,803 • MW: 35.7 kDa
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MERS-CoV Papain-like Protease (recombinant)

Item No. 40988

Product Insert (PDF)Safety Data Sheet (SDS) (PDF)
Technical Information
Synonyms
  • MERS-CoV PLpro
  • Middle East Respiratory Syndrome Coronavirus PLpro
Purity
≥80%
Source
Active recombinant MERS-CoV PLpro expressed in E. coli
Amino Acids
1,480-1,803
MW
35.7 kDa
Formulation (Request formulation change)
50 mM Tris, pH 7.5, with 100 mM sodium chloride, 10% glycerol, and 1 mM DTT
Host
E. coli
Shipping & Storage Information
Storage
-80°C
Shipping
Dry ice in continental US; may vary elsewhere
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    100 µg

    $439

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    Cayman Chemical
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    Product Description

    Middle East respiratory syndrome coronavirus (MERS-CoV) is an enveloped positive-stranded RNA virus, a member of the Betacoronavirus genus, and the causative agent of MERS, an acute respiratory disease that often leads to pneumonia and renal failure.1,2 The MERS-CoV papain-like protease (PLpro) is encoded by the non-structural protein 3 (nsp3) region of ORF1a.3,4 It cleaves non-structural proteins 1-3, which, together with non-structural proteins 4-16, form the replicase complex. In addition, it reverses certain post-translational modifications from host proteins, preferentially removing interferon-stimulated gene product 15 (ISG15) but also removing ubiquitin, with both actions leading to suppression of the host innate immune response.5 MERS-CoV PLpro decreases MDA5-induced mRNA expression of CXCL10, IFNB, and CCL5 and inhibits TNF-α-induced activation of NF-κB in vitro. Cayman’s MERS-CoV Papain-like Protease (recombinant) protein can be used for enzyme activity, surface plasmon resonance (SPR), and Western blot (WB) applications.

    WARNING This product is not for human or veterinary use.

    References & Product Citations
    Product Description References

    1. Du, L., Yang, Y., Zhou, Y., et al. MERS-CoV spike protein: A key target for antivirals. Expert Opin. Ther. Targets 21(2), 131-143 (2017).

    2. Rabaan, A.A., Al-Ahmed, S.H., Haque, S., et al. SARS-CoV-2, SARS-CoV, and MERS-CoV: A comparative overview. Infez. Med. 28(2), 174-184 (2020).

    3. Clasman, J.R., Báez-Santos, Y., Mettelman, R.C., et al. X-ray structure and enzymatic activity profile of a core papain-like protease of MERS coronavirus with utility for structure-based drug design. Sci. Rep. 7, 40292 (2017).

    4. Li, Y.-H., Hu, C.-Y., Wu, N.-P., et al. Molecular characteristics, functions, and related pathogenicity of MERS-CoV proteins. Engineering (Beijing) 5(5), 940-947 (2019).

    5. Mielech, A.M., Kilianski, A., Baez-Santos, Y.M., et al. MERS-CoV papain-like protease has deISGylating and deubiquitinating activities. Virology 450-451, 64-70 (2014).