Z-FF-FMK

Z-FF-FMK is an inhibitor of cathepsin B (K_i = 2.7 nM).¹ It inhibits cell division, cell cycle progression, DNA replication, and chromosome decondensation in isolated T. niger fertilized embryos when used at a concentration of 100 µM.² Z-FF-FMK (10 µM) prevents increases in cathepsin L activity induced by amyloid-β (1-40) (Aβ40), as well as prevents Aβ40-induced apoptosis, DNA fragmentation, and increases in cleaved poly(ADP-ribose) polymerase (PARP) levels, in primary rat cortical neurons.³ In vivo, Z-FF-FMK (5 nmol/animal) inhibits quinolinic acid-induced nuclear translocation of NF-κB, as well as inhibits quinolinic acid-induced increases in phosphorylated IκBα and IKKα levels, in the unilateral striatum in rats.⁴

WARNING This product is not for human or veterinary use.

1. Shoji, A., Suenaga, Y., Hosaka, A., et al. Inhibitory assay for degradation of collagen IV by cathepsin B with a surface plasmon resonance sensor. J. Pharm. Biomed. Anal. 145, 79-83 (2017).

2. Morin, V., Sanchez, A., Quiñones, K., et al. Cathepsin L inhibitor I blocks mitotic chromosomes decondensation during cleavage cell cycles of sea urchin embryos. J. Cell. Physiol. 216(3), 790-795 (2008).

3. Boland, B., and Campbell, V. Abeta-mediated activation of the apoptotic cascade in cultured cortical neurones: A role for cathepsin-L. Neurobiol. Aging 25(1), 83-91 (2004).

4. Wang, Y.R., Qin, S., Han, R., et al. Cathepsin L plays a role in quinolinic acid-induced NF-Κb activation and excitotoxicity in rat striatal neurons. PLoS One 8(9), e75702 (2013).