Mouse Mineralocorticoid Receptor Reporter Assay System

This nuclear receptor assay system utilizes proprietary cells engineered to provide constitutive high-level expression of full-length, Mouse Mineralocorticoid Receptor (nr3c2), a ligand-dependent transcription factor referred to as Mouse MR.

INDIGO's Reporter Cells include the luciferase reporter gene functionally linked to a MR-responsive promoter. Thus, quantifying changes in luciferase expression in the treated reporter cells provides a sensitive surrogate measure of the changes in MR activity. Luciferase gene expression occurs after ligand-bound MR undergoes nuclear translocation, DNA binding, recruitment and assembly of the co-activators and accessory factors required to form a functional transcription complex, culminating in expression of the reporter gene. Unlike some other cell-based assay strategies, the readout from INDIGO's reporter cells demands the same orchestration of all intracellular molecular interactions and events that can be expected to occur in vivo.

Mouse MR Reporter Cells are prepared using INDIGO’s proprietary CryoMite™ process. This cryo-preservation method yields high cell viability post-thaw, and provides the convenience of immediately dispensing healthy, division-competent reporter cells into assay plates. There is no need for cumbersome intermediate treatment steps such as spinand-rinse of cells, viability determinations, or cell titer adjustments prior to assay setup.

INDIGO Bioscience’s Assays are all-inclusive cell-based assay systems. In addition to mouse MR Reporter Cells, this kit provides two optimized media for use during cell culture and in diluting the user's test samples, the reference agonist Aldosterone, Luciferase Detection Reagent, and a cell culture-ready assay plate. [INDIGO Catalog No. M00501]

WARNING This product is not for human or veterinary use.