Host: Insect cells • AA: 291-696 • Tag: C-terminal His • MW: 45.6 kDa
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Zika Virus Envelope Protein (strain Zika SPH2015) (T366A, Q367G, W391R, L397R mutant; recombinant)

Item No. 41068

Product Insert (PDF)
Technical Information
Synonyms
  • Zika Virus E Protein
  • ZIKV E Protein
  • ZIKV Envelope Protein
Purity
≥90% estimated by SDS-PAGE
Endotoxin Testing
<1.0 EU/μg, determined by the LAL endotoxin assay
Source
Recombinant Zika virus C-terminal His-tagged E protein expressed in insect cells
Amino Acids
291-696
MW
45.6 kDa
Formulation (Request formulation change)
Lyophilized from sterile 20 mM Tris, 300 mM sodium chloride, pH 8.0, and 10% glycerol
Host
Insect cells
UniProt Accession №
A0A0U3FSM8
Shipping & Storage Information
Storage
-80°C
Shipping
Dry ice in continental US; may vary elsewhere
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    100 µg

    $383

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    Cayman Chemical
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    Product Description

    Zika virus (ZIKV) is a mosquito-borne, positive-stranded RNA virus and a member of the Flavivirus genus.1,2 ZIKV infection is associated with fever, rashes, and conjunctivitis, as well as more severe symptoms, which include Guillain-Barré syndrome in adults and microcephaly or congenital malformations in fetuses and newborns.1,3 The single-stranded RNA genome of ZIKV is translated as a polypeptide, which is cleaved by host and viral proteases into structural capsid (C), precursor membrane (prM), and envelope (E) proteins and seven non-structural proteins: NS1, NS2A, NS2B, NS3, NS4A, NS4B, and NS5.1,4 ZIKV E protein is composed of a structural domain, a dimerization domain, which contains a membrane fusion loop, and a structural domain containing a hinge region, two transmembrane helices, and a host receptor-binding region.2 It is a homodimer in the viral membrane but transitions to a homotrimer state in host cell organelles with low pH, in which viral replication and assembly occurs, as well as where E protein can form a heterodimer with prM.4 ZIKV E protein glycosylation at an N-terminal asparagine is necessary for ZIKV infectivity and optimal replication in cells and mice.3 Cayman's Zika Virus Envelope Protein (strain Zika SPH2015) (T366A, Q367G, W391R, L397R mutant; recombinant) protein consists of 405 amino acids, has a calculated molecular weight of 45.6 kDa, and a predicted N-terminus of Ile291 after signal peptide cleavage.

    WARNING This product is not for human or veterinary use.

    References & Product Citations
    Product Description References

    1. Lei, J., Hansen, G., Nitsche, C., et al. Crystal structure of Zika virus NS2B-NS3 protease in complex with a boronate inhibitor. Science 353(6298), 503-505 (2016).

    2. Hu, T., Wu, Z., Wu, S., et al. The key amino acids of E protein involved in early flavivirus infection: Viral entry. Virol. J. 18(1), 136 (2021).

    3. Carbaugh, D.L., Baric, R.S., and Lazear, H.M. Envelope protein glycosylation mediates Zika virus pathogenesis. J. Virol. 93(12), e00113-e00119 (2019).

    4. Dai, L., Song, J., Lu, X., et al. Structures of the Zika virus envelope protein and its complex with a flavivirus broadly protective antibody. Cell Host Microbe. 19(5), 696-704 (2016).