TEAD2 Cofactor-Binding Domain (human, recombinant)

TEA domain transcription factor 2 (TEAD2) is a transcription factor and member of the TEA domain-containing family of transcription factors.¹ It is composed of an N-terminal TEA DNA-binding domain, a hydrophobic proline-rich domain, and a C-terminal cofactor-binding domain (CBD), which binds TAZ or YES-associated transcriptional regulator (YAP), or vestigial-like proteins (VGLLs), and is involved in TAZ-, YAP-, or VGLL-dependent transcription of genes in the Hippo signaling pathway, which regulates embryonic and organ development, proliferation, and cell death. ^1,2,3 The tertiary structure and function of TEAD2 requires C-terminal palmitoylation at residue 380.⁴ TEAD2 binds to the promoter for Pou5f1, the gene encoding octamer-binding transcription factor 3 (Oct3), and is necessary for mouse embryonic stem cell self-renewal.⁵ Increased tumor levels of TEAD2 mRNA are associated with poor survival, angiogenesis, tumor progression, and increased expression of genes associated with the epithelial-to-mesenchymal transition (EMT) in patients with hepatocellular carcinoma (HCC).⁶ Cayman's TEAD2 Cofactor-Binding Domain (human, recombinant) protein consists of 230 amino acids, has a calculated molecular weight of 26.4 kDa, and is depalmitoylated at cysteine 380.

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1. Landin-Malt, A., Benhaddou, A., Zider, A., et al. An evolutionary, structural and functional overview of the mammalian TEAD1 and TEAD2 transcription factors. Gene 591(1), 292-303 (2016).

2. Tian, W., J., Y., Tomchick, D.R., et al. Structural and functional analysis of the YAP-binding domain of human TEAD2. Proc. Natl. Acad. Sci. U S A 107(16), 7293-7298 (2010).

3. Hillen, H., Candi, A., Vanderhoydonck, B., et al. A novel irreversible TEAD inhibitor, SWTX-143, blocks hippo pathway transcriptional output and causes tumor regression in preclinical mesothelioma models. Mol. Cancer Ther. 23(1), 3-13 (2023).

4. Noland, C.L., Gierke, S., Schnier, P.D., et al. Palmitoylation of TEAD transcription factors is required for their stability and function in Hippo pathway signaling. Structure 24(1), 179-186 (2016).

5. Tamm, C., Böwer, N., and Annerén, C. Regulation of mouse embryonic stem cell self-renewal by a Yes-YAP-TEAD2 signaling pathway downstream of LIF. J. Cell Sci. 124(Pt 7), 1136-1144 (2011).

6. Joo, J.S., Cho, S.Y., Rou, W.S., et al. TEAD2 as a novel prognostic factor for hepatocellular carcinoma. Oncol. Rep. 43(6), 1785-1796 (2020).