Host: Insect cells • AA: 2,772-3,423 • Tag: C-terminal His • MW: 76.7 kDa
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Zika Virus NS5 Protein (strain Zika SPH2015) (recombinant)

Item No. 41079

Product Insert (PDF)
Technical Information
Synonyms
  • ZIKV-NS5
  • ZIKV Non-structural Protein 5
Purity
≥85% estimated by SDS-PAGE
Endotoxin Testing
<1.0 EU/µg determined by the LAL endotoxin assay
Source
Recombinant Zika virus C-Terminal His-tagged NS5 (strain Zika SPH2015) expressed in insect cells
Amino Acids
2,772-3,423
MW
76.7 kDa
Formulation (Request formulation change)
Sterile 20 mM PB,100 mM sodium chloride, 50% glycerol, pH 7.0, 0.5 mM TCEP
UniProt Accession №
A0A0U3FSM8
Shipping & Storage Information
Storage
-80°C
Shipping
Dry ice in continental US; may vary elsewhere
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    100 µg

    $383

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    Cayman Chemical
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    Product Description

    Zika virus (ZIKV) is a mosquito-borne, positive-stranded RNA virus and a member of the Flavivirus genus.1,2 ZIKV infection is associated with fever, rashes, and conjunctivitis, as well as more severe symptoms, which include Guillain-Barré syndrome in adults and microcephaly or congenital malformations in fetuses and newborns.1,3 The single-stranded RNA genome of ZIKV is translated as a polypeptide, which is cleaved by host and viral proteases into structural capsid (C), precursor membrane (prM), and envelope (E) proteins and seven non-structural proteins: NS1, NS2A, NS2B, NS3, NS4A, NS4B, and NS5.1,4 ZIKV NS5 is composed of an N-terminal methyltransferase (MTase) domain involved in 5’ RNA capping prior to translation, a short linker region, and a C-terminal RNA-dependent RNA polymerase (RdRp) domain required for viral RNA replication.5,6,7 ZIKV NS5 inhibits host IFN signaling by binding to the human, but not mouse, transcriptional activator STAT2 and targeting it for proteasomal degradation.8,7 Cayman's Zika virus NS5 (strain Zika SPH2015) (recombinant) protein consists of 663 amino acids and has a calculated molecular weight of 76.7 kDa. By SDS-PAGE, under reducing conditions, the apparent molecular mass of the protein is approximately 64 kDa.

    WARNING This product is not for human or veterinary use.

    References & Product Citations
    Product Description References

    1. Lei, J., Hansen, G., Nitsche, C., et al. Crystal structure of Zika virus NS2B-NS3 protease in complex with a boronate inhibitor. Science 353(6298), 503-505 (2016).

    2. Hu, T., Wu, Z., Wu, S., et al. The key amino acids of E protein involved in early flavivirus infection: Viral entry. Virol. J. 18(1), 136 (2021).

    3. Carbaugh, D.L., Baric, R.S., and Lazear, H.M. Envelope protein glycosylation mediates Zika virus pathogenesis. J. Virol. 93(12), e00113-e00119 (2019).

    4. Dai, L., Song, J., Lu, X., et al. Structures of the Zika virus envelope protein and its complex with a flavivirus broadly protective antibody. Cell Host Microbe. 19(5), 696-704 (2016).

    5. Zhao, B., Yi, G., Du, F., et al. Structure and function of the Zika virus full-length NS5 protein. Nat. Commun. 8, 14762 (2017).

    6. Shi, Y., and Geo, G.F. Structural biology of the zika virus. Trends Biochem. Sci. 42(6), 443-456 (2017).

    7. Wang, B., Thurmond, S., Hai, R., et al. Structure and function of Zika virus NS5 protein: Perspectives for drug design. Cell Mol. Life Sci. 75(10), 1723-1736 (2018).

    8. Grant, A., Ponia, S.S., Tripathi, S., et al. Zika virus targets human STAT2 to inhibit type I interferon signaling. Cell Host Microbe 19(6), 882-890 (2016).