Cell-free Protein Synthesis (E. coli) Kit

Cell-free protein synthesis (CFPS) is an easy-to-use, rapid, and efficient system that is devoid of membrane-bound barriers but contains the native cellular transcriptional and translational machinery and exogenous resources (e.g., amino acids, nucleotides, and a secondary energy substrate) needed for synthesis of the desired proteins.^1,2,3,4,5

The CFPS (E. coli) Kit can produce high levels and different molecular weights of recombinant proteins within several hours and can be used in high-throughput functional genomics and proteomics. In addition, it can provide proteins to drive biochemical and structural biology research.

The synthesized protein can be detected by SDS-PAGE, Western blot, and fluorescence-detection size exclusion chromatography (FSEC) and can be isolated from the reaction mixture by affinity purification techniques for further structural and/or functional characterization.

WARNING This product is not for human or veterinary use.

1. Silverman, A.D., Karim, A.S., and Jewett, M.C. Cell-free gene expression: An expanded repertoire of applications. Nat. Rev. Genet. 21(3), 151-170 (2020).

2. Garenne, D., Haines, M.C., Romantseva, E., et al. Cell-free gene expression. Nat. Rev. 1, 49 (2021).

3. Jewett, M.C., and Swartz, J.R. Mimicking the Escherichia coli cytoplasmic environment activates long-lived and efficient cell-free protein synthesis. Biotechnol. Bioeng. 86(1), 19-26 (2004).

4. Davanloo, P., Rosenberg, A.H., Dunn, J.J., et al. Cloning and expression of the gene for bacteriophage T7 RNA polymerase. Proc. Nat. Acad. Sci. USA 81(7), 2035-2039 (1984).