A fluorogenic substrate for chymotrypsin
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Chymotrypsin Substrate II, Fluorogenic

Item No. 41593

Product Insert (PDF)Safety Data Sheet (SDS) (PDF)
Technical Information
Formal Name
N-(3-carboxy-1-oxopropyl)-L-alanyl-L-alanyl-L-prolyl-N-(4-methyl-2-oxo-2H-1-benzopyran-7-yl)-L-phenylalaninamide
CAS Number
88467-45-2
Synonyms
  • Suc-AAPF-AMC
  • Suc-Ala-Ala-Pro-Phe-AMC
  • Suc-Ala2-Pro-Phe-AMC
  • Succinyl-Ala-Ala-Pro-Phe-AMC
Molecular Formula
C34H39N5O9
Formula Weight
661.7
Purity
≥98%
Emission
440-460 nm
Excitation
340-360 nm
Formulation (Request formulation change)
A solid
Solubility(Learn about Variance in Solubility)
DMSO: Soluble: ≥10 mg/mlEthanol: Soluble: ≥10 mg/ml
SMILES
O=C([C@H](C)NC([C@H](C)NC(CCC(O)=O)=O)=O)N1CCC[C@H]1C(N[C@H](C(NC2=CC=C(C(C)=CC(O3)=O)C3=C2)=O)CC4=CC=CC=C4)=O
InChi Code
InChI=1S/C34H39N5O9/c1-19-16-30(43)48-27-18-23(11-12-24(19)27)37-32(45)25(17-22-8-5-4-6-9-22)38-33(46)26-10-7-15-39(26)34(47)21(3)36-31(44)20(2)35-28(40)13-14-29(41)42/h4-6,8-9,11-12,16,18,20-21,25-26H,7,10,13-15,17H2,1-3H3,(H,35,40)(H,36,44)(H,37,45)(H,38,46)(H,41,42)/t20-,21-,25-,26-/m0/s1
InChi Key
FMVUZZZUYBXQGR-KCXKOMAXSA-N
Shipping & Storage Information
Storage
-20°C
Shipping
Wet ice in continental US; may vary elsewhere
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    5 mg

    $35

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    10 mg

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    25 mg

    $154

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    50 mg

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    Product Description

    Chymotrypsin substrate II, fluorogenic is a fluorogenic substrate for chymotrypsin and chymotrypsin-like proteases.1,2 Upon enzymatic cleavage by proteases, 7-amino-4-methylcoumarin (AMC) is released and its fluorescence can be used to quantify protease activity. AMC displays excitation/emission maxima of 340-360/440-460 nm, respectively.

    WARNING This product is not for human or veterinary use.

    References & Product Citations
    Product Description References

    1. Oshima, G. Interaction of α-chymotrypsin with dimyristoyl phosphatidylcholine vesicles. J. Biochem. 95(4), 1131-1136. (1984).

    2. Irvine, G.B., Ennis, M., and Williams, C.H. Visual detection of peptidase activity using fluorogenic substrates in a microtiter plate assay. Anal. Biochem. 185(2), 304-307 (1990).