Naveni PD1/PD-L1 BOND RX HRP

The Naveni^® PD1/PD-L1 BOND RX HRP is an in situ proximity ligation assay kit for the detection of the interaction of PD1 and PD-L1 using BOND RX/RX^m Fully Automated Research Stainer. The kit has been validated on FFPE human tissue samples.

The kit is based on the Naveni^® in situ proximity ligation technology with an automated workfflow. It uses two Navenibodies linked to proprietary oligo arms. The primary antibodies included in the kit bind to PD1 and PD-L1 proteins, while Navenbodies then bind to their respective primary antibodies. When Navenbodies are in close proximity, a strong and distinct signal is generated, offering a specific and sensitive detection method for the interaction between PD1 and PD-L1. Read more about Naveni technology.

Included in the kit:
• Navenibody targeting human PD1 protein based on clone EH33 CST
• Navenibody targeting human PD-L1 protein based on clone SP142 Abcam RabMAb^®
• Buffers for blocking and dilutions, and all detection reagents for the PD1/PD-L1 interaction signal
• Reagents sufficient for 30 FFPE tissue slides, including dead volumes

Not included in the kit:
• Leica Biosystems for BOND RX/RX^m with standard accessories. • Mounting medium

Find better prognostic biomarkers
PD1/PD-L1 signaling has with immune checkpoint inhibitors proven to be significant in cancer progression and treatment. Despite the recent success of immune checkpoint inhibitors, many patients do not benefit from these therapies, and predictive biomarkers improving patient stratification are needed. PD-L1 IHC is commonly used as a biomarker, but the correlation between PD-L1 expression levels and PD1/PD-L1 interaction is not alwyas linear.

Naveni^® PPI
Proteins perform a wide range of functions and are the cloest way to understand biology. Proteins are also key targets in both health and disease. When proteins carry out their function, they interact with other proteins or molecules. To understand biological pathways and mode of action, we need to get a deeper insight into protein functions and how the protein interacts. Detecting the interactions has until now been either complicated, unspecific, or insensitive. The Naveni^® in situ proximity ligation technology enables visualization of protein-protein interaction using one primary antibody for each target protein, without the need to disrupt the tissue microenvironment.

WARNING This product is not for human or veterinary use.