Ubiquitin-like-specific Protease 1 (S. cerevisiae, recombinant)

Ubiquitin-like-specific protease 1 (Ulp1) is a small ubiquitin-related modifier (SUMO) protease and member of the cysteine protease superfamily.¹ It is composed of an N-terminal regulatory domain and a C-terminal protease domain.² Ulp1 processes Smt3, the yeast ortholog to human SUMO, into its mature form by cleavage of the alanine-threonine-tyrosine residues at the C-terminal end.¹ It also deconjugates Smt3 from Smt3-protein conjugates, which is required for normal cell cycle progression in S. cerevisiae, and can cleave human SUMO-tagged proteins.^3,2,1 A version of Ulp1 lacking the N-terminal regulatory domain does not localize to the nuclear pore complex and can desumoylate certain Ulp2 targets. Cayman’s Ubiquitin-like-specific Protease 1 (S. cerevisiae, recombinant) protein can be used for SUMO tag cleavage from SUMO fusion proteins.

WARNING This product is not for human or veterinary use.

1. Mossessova, E., and Lima, C.D. Ulp1-SUMO crystal structure and genetic analysis reveal conserved interactions and a regulatory element essential for cell growth in yeast. Mol. Cell 5(5), 865-876 (2000).

2. Li, S.-J., and Hochstrasser, M. The Ulp1 SUMO isopeptidase: distinct domains required for viability, nuclear envelope localization, and substrate specificity. J. Cell. Biol. 160(7), 1069-1081 (2003).

3. Li, S.-J., and Hochstrasser, M. A new protease required for cell-cycle progression in yeast. Nature 398(6724), 246-251 (1999).