A fluorescent probe
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Fluorescent Brightener 28

Item No. 41995

Product Insert (PDF)Safety Data Sheet (SDS) (PDF)
Technical Information
Formal Name
2,2′-(1,2-ethenediyl)bis[5-[[4-[bis(2-hydroxyethyl)amino]-6-(phenylamino)-1,3,5-triazin-2-yl]amino]-benzenesulfonic acid, disodium
CAS Number
4193-55-9
Synonyms
  • Calcofluor White LRP
  • Calcofluor White M2R
  • Calcofluor White ST
  • Cellufluor
  • CFW
  • C.I. 40622
  • FB28
  • Fluostain I
  • UNPA-GX
Molecular Formula
C40H42N12O10S2 • 2Na
Formula Weight
960.9
Emission
430 nm
Excitation
360 nm
Formulation (Request formulation change)
A solid
Solubility(Learn about Variance in Solubility)
Methanol: Sparingly soluble: 1-10 mg/ml
λmax
211, 245, 267, 356 nm
SMILES
OCCN(C1=NC(NC2=CC=CC=C2)=NC(NC(C=C3)=CC([S](=O)([O-])=O)=C3C=CC(C=CC(NC4=NC(NC5=CC=CC=C5)=NC(N(CCO)CCO)=N4)=C6)=C6[S](=O)([O-])=O)=N1)CCO.[Na+].[Na+]
InChi Code
InChI=1S/C40H44N12O10S2.2Na/c53-21-17-51(18-22-54)39-47-35(41-29-7-3-1-4-8-29)45-37(49-39)43-31-15-13-27(33(25-31)63(57,58)59)11-12-28-14-16-32(26-34(28)64(60,61)62)44-38-46-36(42-30-9-5-2-6-10-30)48-40(50-38)52(19-23-55)20-24-56;;/h1-16,25-26,53-56H,17-24H2,(H,57,58,59)(H,60,61,62)(H2,41,43,45,47,49)(H2,42,44,46,48,50);;/q;2*+1/p-2
InChi Key
YJHDFAAFYNRKQE-UHFFFAOYSA-L
Shipping & Storage Information
Storage
-20°C
Shipping
Room temperature in continental US; may vary elsewhere
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    250 mg

    $32

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    500 mg

    $61

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    1 g

    $115

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    5 g

    $256

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    Product Description

    Fluorescent brightener 28 is a fluorescent probe.1 It has been used to image chitin in pale grass blue butterfly (Z. maha) wings and β-glucans and glycans in the cell walls of cucumber plant stem and root tissues.2,3,1 Fluorescent brightener 28 displays an emission maximum of 430 nm upon excitation at 360 nm.1 Formulations containing fluorescent brightener 28 have been used as brightening agents in soap, paper, and cotton fabrics.

    WARNING This product is not for human or veterinary use.

    References & Product Citations
    Product Description References

    1. Piccinini, L., Ramamonjy, F.N., and Ursache, R. Imaging plant cell walls using fluorescent stains: The beauty is in the details. J. Microsc. 295(2), 102-120 (2024).

    2. Nakazato, Y., and Otaki, J.M. Live detection of intracellular chitin in butterfly wing epithelial cells in vivo using fluorescent brightener 28: Implications for the development of scales and color patterns. Insects 14(9), 753 (2023).

    3. Schnieder, N., Känel, A., Zimmermann, M., et al. So similar yet so different: The distinct contributions of extrafascicular and fascicular phloem to transport and exudation in cucumber plants. J. Plant Physiol. 271, 153643 (2022).