Host: Insect cells • AA: 39-426 • Tag: N-terminal His • MW: 48.2 kDa
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Cbl-b Substrate-binding Domain (human, recombinant)

Item No. 41999

Product Insert (PDF)
Technical Information
Synonyms
  • Casitas B-lineage Lymphoma Proto-oncogene B
  • Casitas B Lymphoma-B
  • RING Finger Protein 56
  • RING-type E3 Ubiquitin Transferase Cbl-b
  • RNF56
  • SH3-binding Protein Cbl-b
  • Signal Transduction Protein Cbl-b
  • E3 Ubiquitin-protein Ligase Cbl-b
Source
Recombinant human N-terminal His-tagged Cbl-b substrate-binding domain expressed in insect cells
Amino Acids
39-426
MW
48.2 kDa
Formulation (Request formulation change)
50 mM Tris-HCl (pH 7.5), with 200 mM sodium chloride, 20% glycerol, and 1 mM DTT
UniProt Accession №
Q13191
Shipping & Storage Information
Storage
-80°C
Shipping
Dry ice in continental US; may vary elsewhere
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Certificates of Analysis & Batch Specific Data

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    100 µg

    $1,026

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    500 µg

    $3,540

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    Cayman Chemical
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    Product Description

    Casitas B-lineage lymphoma proto-oncogene B (Cbl-b) is an E3 ubiquitin ligase and a member of Cbl RING-type E3 ubiquitin ligases.1,2 It is composed of an N-terminal tyrosine kinase-binding domain, a helix linker region, and a RING finger domain, which is necessary for Cbl-b homodimerization and heterodimerization with the related homolog c-Cbl, and a C-terminal region containing proline- and tyrosine-rich motifs and a ubiquitin-association domain.2 Cbl-b is autoinhibited by its helix linker region but folds into the active conformation following tyrosine 371 phosphorylation by various kinases.3 Cbl-b is ubiquitously expressed but primarily found in leukocytes and is found in the cytoplasm.1 It has roles in negatively regulating immune cell responses, preventing autoimmune activity, and promoting immune tolerance by targeting receptor and non-receptor tyrosine kinase signaling proteins for proteasomal degradation.1,4 Knockout of Cblb increases the percentage of natural killer (NK) cells expressing IFN-γ, as well as decreases tumor volume and number of total metastases, in a B16/F10 murine melanoma model of metastasis.4 Knockout of Cblb increases serum IgG levels, as well as induces Cd28-independent lymphocyte hyperproliferation and B and T cell infiltration into the pancreas, salivary glands, and lungs, in mice.5 Cbl-b levels are decreased in T cell lymphocytes isolated from patients with systemic lupus erythematosus (SLE).6 Cayman's Cbl-b Substrate-binding Domain (human, recombinant) protein has a calculated molecular weight of 48.2 kDa.

    WARNING This product is not for human or veterinary use.

    References & Product Citations
    Product Description References

    1. Lutz-Nicoladoni, C., Wolf, D., and Sopper, S. Modulation of immune cell functions by the E3 ligase Cbl-b. Front. Oncol. 5, 58 (2015).

    2. Kimani, S.W., Perveen, S., Szewezyk, M., et al. The co-crystal structure of Cbl-b and a small-molecule inhibitor reveals the mechanism of Cbl-b inhibition. Commun. Biol. 6(1), 1272 (2023).

    3. Buetow, L., Tria, G., Ahmed, S.F., et al. Casitas B-lineage lymphoma linker helix mutations found in myeloproliferative neoplasms affect conformation. BMC Biol. 14(1), 76 (2016).

    4. Paolino, M., Choidas, A., Wallner, S., et al. The E3 ligase Cbl-b and TAM receptors regulate cancer metastasis via natural killer cells. Nature 507(7493), 508-512 (2014).

    5. Bachmaier, K., Krawczyk, C., Kozieradzki, I., et al. Negative regulation of lymphocyte activation and autoimmunity by the molecular adaptor Cbl-b. Nature 403(6766), 211-216 (2000).

    6. Doníz-Padilla, L., Martínez-Jiménez, V., Niño-Moreno, P., et al. Expression and function of Cbl-b in T cells from patients with systemic lupus erythematosus, and detection of the 2126 A/G Cblb gene polymorphism in the Mexican mestizo population. Lupus 20(6), 628-635 (2011).