Host: E. coli • AA: 96-628 • Tag: N-terminal His- and SUMO-tagged • MW: 73.86 kDa
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ASH2L (human, recombinant)

Item No. 42063

Product Insert (PDF)Safety Data Sheet (SDS) (PDF)
Technical Information
Synonyms
  • Absent, Small, or Homeotic Discs 2-like
  • ASH2-like, Histone Lysine Methyltransferase Complex Subunit
  • Set1/Ash2 Histone Methyltransferase Complex Subunit Ash2 Isoform A
Purity
≥70%
Source
Recombinant human N-terminal His- and SUMO-tagged ASH2L expressed in E. coli
Amino Acids
96-628
MW
73.86 kDa
Formulation (Request formulation change)
PBS, pH 7.5, with 20% glycerol
UniProt Accession №
Q9UBL3
Shipping & Storage Information
Storage
-80°C
Shipping
Dry ice in continental US; may vary elsewhere
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    50 µg

    $402

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    Product Description

    ASH2-like, histone lysine methyltransferase complex subunit (ASH2L) is a member of the trithorax group (TrxG) of proteins and an obligatory component of all histone H3K4 activating methylation complexes, also known as COMPASS complexes.1,2,3 It is ubiquitously expressed and composed of a PHD finger, a WH motif, an SPRY domain, and a dpy-30 histone methyltransferase complex regulatory subunit (DPY30) binding motif.4,5 ASH2L, in conjunction with WD repeat domain 5 (WDR5), RB-binding protein 5 (RBBP5), and DPY30, optimizes the methyltransferase activity of histone-lysine N-methyltransferase SET enzymes within COMPASS complexes and has roles in epigenetic regulation and neurogenesis.3 Global knockdown of Ash2l induces early embryonic lethality in mice. Dorsal ectoderm-targeted knockdown of ash2l in late, but not early, Xenopus neurula embryos inhibits neural and neural crest marker gene expression. ASH2L levels are increased in acute myeloid leukemia (AML) cells isolated from patients with mutant FMS-related tyrosine kinase 3 (FLT-3) compared with those isolated from patients with wild-type FLT-3, and lower levels of ASH2L are positively correlated with increased overall survival.5 Cayman’s ASH2L (human, recombinant) protein can be used for Western blot (WB) applications.

    WARNING This product is not for human or veterinary use.

    References & Product Citations
    Product Description References

    1. Wang, J., Zhou, Y., Yin, B., et al. ASH2L: Alternative splicing and downregulation during induced megakaryocytic differentiation of multipotential leukemia cell lines. J. Mol. Med. (Berl.) 79(7), 399-405 (2001).

    2. Steward, M.M., Lee, J.S., O'Donovan, A., et al. Molecular regulation of H3K4 trimethylation by ASH2L, a shared subunit of MLL complexes. Nat. Struct. Mol. Biol. 13(9), 852-854 (2009).

    3. Mohammadparast, S., and Chang, C. Ash2l, an obligatory component of H3K4 methylation complexes, regulates neural crest development. Dev. Biol. 492, 14-24 (2022).

    4. Chen, Y., Cao, F., Wan, B., et al. Structure of the SPRY domain of human Ash2L and its interactions with RbBP5 and DPY30. Cell Res. 22(3), 598-602 (2012).

    5. Butler, J.S., Qiu, Y.H., Zhang, N., et al. Low expression of ASH2L protein correlates with a favorable outcome in acute myeloid leukemia. Leuk. Lymphoma 58(5), 1207-1218 (2017).