DBI (human, recombinant)

Diazepam-binding inhibitor (DBI), also known as acyl-CoA-binding protein 1a (ACBP-1a), is a lipid-binding protein and ligand of the mitochondrial benzodiazepine receptor.^1,2,3 It is composed of an ACBP domain, which binds saturated and unsaturated medium- and long-chain acyl-CoAs.^1,2,4 DBI undergoes extensive alternative splicing, is ubiquitously expressed, and localizes to the nucleus, cytoplasm, endoplasmic reticulum, and Golgi.^5,6,7 It is involved in acyl-CoA transport and autophagy, and its expression is regulated by peroxisome proliferator-activated receptor γ (PPARγ) and sterol regulatory element binding proteins (SREBPs).^4,5,8,9 Recombinant DBI reduces fatty acid oxidation and glycemia and increases food intake in mice.⁸ Plasma levels of DBI are decreased in patients with anorexia nervosa and increased in patients with obesity. The SNP variant DBI^V88M is associated with anxiety with panic attacks.¹⁰ Cayman’s DBI (human, recombinant) protein consists of 122 amino acids and has a calculated molecular weight of 14 kDa. It migrates as an approximately 18 KDa band in SDS-PAGE under reducing conditions.

WARNING This product is not for human or veterinary use.

1. Decker, C., Miro Obradors, M.J., Sillence, D.J., et al. Phorbol ester-sensitive phospholipase D is mainly localized in the endoplasmic reticulum of BHK cells. Biochem. J. 320(Pt 3 (Pt 3), 885-890 (1996).

2. Gallego, S.F., Sporenger, R.R., Neess, D., et al. Quantitative lipidomics reveals age-dependent perturbations of whole-body lipid metabolism in ACBP deficient mice. Biochim. Biophys. Acta 1862(2), 145-155 (2017).

3. James, M.L., Selleri, S., and Kassiou, M. Development of ligands for the peripheral benzodiazepine receptor. Curr. Med. Chem. 13(17), 1991-2001 (2006).

4. Rasmussen, J.T., Faergeman, N.J., Kristiansen, K., et al. Acyl-CoA-binding protein (ACBP) can mediate intermembrane acyl-CoA transport and donate acyl-CoA for β-oxidation and glycerolipid synthesis. Biochem. J. 299(Pt 1), 165-170 (1994).

5. Nitz, I., Döring, F., Schrezenmeir, J., et al. Identification of new acyl-CoA binding protein transcripts in human and mouse. Int. J. biochem. Cell Biol. 37(11), 2395-2405 (2005).

6. Nitz, I., Kruse, M.-L., Klapper, M., et al. Specific regulation of low-abundance transcript variants encoding human Acyl-CoA binding protein (ACBP) isoforms. J. Cell Mol. Med. 15(4), 909-927 (2011).

7. Hansen, J., Faergeman, N.J., Kragelund, B.B., et al. Acyl-CoA-binding protein (ACBP) localizes to the endoplasmic reticulum and Golgi in a ligand-dependent manner in mammalian cells. Biochem. J. 410(3), 463-472 (2008).

8. Pedro, J.M.B.-S., Sica, V., Martins, I., et al. Acyl-CoA-binding protein is a liogenic factor that triggers food intake and obesity. Cell Metab. 30(4), (2019).

9. Ettinger, S.L., Sobel, R.A., Whitmore, T.G., et al. Dysregulation of sterol response element-binding proteins and downstream effectors in prostate cancer during progression to androgen independence. Cancer Res. 64(6), 2212-2221 (2004).

10. Thoeringer, C.K., Binder, E.B., Salyakina, D., et al. Association of a Met88Val diazepam binding inhibitor (DBI) gene polymorphism and anxiety disorders with panic attacks. J. Psychiatr. Res. 41(7), 579-584 (2007).