LOXL2 (human, recombinant)

Lysyl oxidase-like 2 (LOXL2) is a copper-dependent amine oxidase and member of the LOX family.¹ It is composed of four N-terminal scavenger receptor cysteine-rich (SRCR) domains and a conserved C-terminal catalytic domain containing a copper-binding site.^2,1 LOXL2 is secreted by fibroblasts and reproductive epithelial cells and oxidizes lysines to reactive aldehydes to cross-link extracellular matrix (ECM) proteins, such as collagen or elastin.¹ It has roles in connective tissue development, ECM remodeling, fibrosis, and cancer invasion and metastasis. It also oxidizes dimethylated lysine 4 on histone H3 (H3K4me2) and H3K4me3 and reduces the levels of E-cadherin in vitro.³ The levels of LOXL2 are increased in the airway epithelium in a mouse model of ovalbumin-induced asthma and in patients with asthma.⁴ Hepatocyte levels of LOXL2 are increased in patients with Wilson's disease or primary biliary cirrhosis and in patients infected with hepatitis B or -C.⁵ Cayman's LOXL2 (human, recombinant) protein can be used for enzyme activity assays. This protein has a calculated molecular weight of 85.5 kDa and a predicted N-terminus of Gly26 after signal peptide cleavage. By SDS-PAGE, under reducing conditions, the apparent molecular mass of the protein is 110-116 kDa due to glycosylation.

WARNING This product is not for human or veterinary use.

1. Cano, A., Eraso, P., Mazón, M.J., et al. LOXL2 in cancer: A two-decade perspective. Int. J. Mol. Sci. 24(18), 14405 (2023).

2. Zhang, X., Wang, Q., Wu, J., et al. Crystal structure of human lysyl oxidase-like 2 (hLOXL2) in a precursor state. Proc. Natl. Acad. Sci. USA 115(5), 3828-3833 (2018).

3. Herranz, N., Dave, N., Millanes-Romero, A., et al. Lysyl oxidase-like 2 (LOXL2) oxidizes trimethylated lysine 4 in histone H3. FEBS J. 283(23), 4263-4273 (2016).

4. Zeng, R., Zhang, D., Zhang, J., et al. Targeting lysyl oxidase like 2 attenuates OVA-induced airway remodeling partly via the AKT signaling pathway. Respir. Res. 25(1), 230 (2024).

5. Vadasz, Z., Kessler, O., Akiri, G., et al. Abnormal deposition of collagen around hepatocytes in Wilson’s disease is associated with hepatocyte specific expression of lysyl oxidase and lysyl oxidase like protein-2. J. Hepatol. 43(3), 499-507 (2005).