Active • Host: E. coli • AA: Met1; 139-257 • MW: 13.8 kDa
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NT-3 (human, recombinant)

Item No. 42755

Product Insert (PDF)
Technical Information
Synonyms
  • HDNF
  • Hippocampus-derived Neurotrophic Factor
  • Nerve Growth Factor 2
  • Neurotrophin-3
  • NGF-2
  • NTF3
Purity
≥90% estimated by SDS-PAGE
Source
Active recombinant human NT-3 expressed in E. coli
Amino Acids
Met1; 139-257
MW
13.8 kDa
Formulation (Request formulation change)
Lyophilized from sterile PBS, pH 5.2, 5% trehalose, 5% mannitol, and 0.01% Tween 80
UniProt Accession №
P20783
Shipping & Storage Information
Storage
-80°C
Shipping
Dry ice in continental US; may vary elsewhere
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    20 µg

    $196

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    100 µg

    $402

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    Product Description

    Neurotrophin-3 (NT-3) is a neurotrophin that is involved in neurogenesis and neuronal plasticity, proliferation, differentiation, and survival.1,2,3 It is produced as a precursor protein composed of a signal peptide, a prodomain, and the mature NT-3 sequence, which is secreted from neurons after cleavage of the proprotein.2 NT-3 is expressed in the cerebellum and peripheral tissues such as the heart, spleen, and kidneys.4,5 It homodimerizes and induces neurotropic signaling mainly through tropomyosin-related kinase C (TrkC) and the p75 neurotrophin receptor (p75NTR), but it does signal through TrkA and TrkB to a lesser extent.1,2 NT-3 induces neurite outgrowth in chick embryonic peripheral sensory and sympathetic neurons in vitro.5 Conditional knockout of Ntf3, the gene encoding NT-3, in the brain induces learning deficits in a spatial memory task in mice.3 Hippocampal and prefrontal cortex levels of NT-3 are decreased in suicide victims.6 Cayman’s NT-3 (human, recombinant) protein can be used for binding assays. The protein was synthesized from a DNA sequence encoding the mature form of human NT-3 (Tyr139-Thr257) with an N-terminal translation-initiating methionine (Met1). The expressed protein consists of 120 amino acids, has a calculated molecular weight of 13.8 kDa, and a predicted N-terminus of Met1.

    WARNING This product is not for human or veterinary use.

    References & Product Citations
    Product Description References

    1. Davies, A.M. Neurotrophins: The yin and yang of nerve growth factor. Curr. Biol. 7(1), R38-R40 (1997).

    2. Bothwell, M. NGF, BDNF, NT3, and NT4. Neurotrophic Factors. Handbook of Experimental Pharmacology 220, 3-15 (2014).

    3. Shimazu, K., Zhao, M., Sakata, K., et al. NT-3 facilitates hippocampal plasticity and learning and memory by regulating neurogenesis. Learn. Mem. 13(3), 307-315 (2006).

    4. Jones, K.R., and Reichardt, L.F. Molecular cloning of a human gene that is a member of the nerve growth factor family. Proc. Natl. Acad. Sci. USA 87(20), 8060-8064 (1990).

    5. Rosenthal, A., Goeddel, D.V., Nguyen, T., et al. Primary structure and biological activity of a novel human neurotrophic factor. Neuron 4(5), 767-773 (1990).

    6. Karege, F., Vaudan, G., Schwald, M., et al. Neurotrophin levels in postmortem brains of suicide victims and the effects of antemortem diagnosis and psychotropic drugs. Brain Res. Mol. Brain Res. 136(1-2), 29-37 (2005).