Angiopoietin-1 (human, recombinant)

Angiopoietin-1 (ANG-1) is a member of the angiopoietin family of growth factors.^1,2 It is composed of a superclustering domain, which mediates oligomerization, a coiled-coil domain, and a fibrinogen-like domain, which facilitates receptor binding. ANG-1 is ubiquitously expressed and forms homomultimers, which are required for activation of its receptors, tunica interna endothelial cell kinase 2 (Tie2) and Tie1. It plays a role in angiogenesis, vascular remodeling and permeability, inflammation, hematopoiesis, and inhibition of apoptosis in endothelial cells. Mutations in ANGPT1, the gene encoding ANG-1, are associated with hereditary angioedema.³ Increased protein levels of ANG-1 correlate with increased levels of Tie2 in patients with colorectal cancer.⁴ Cayman’s Angiopoietin-1 (human, recombinant) protein is a disulfide-linked homodimer. The reduced monomer, composed of ANG-1 (amino acids 16-261 and 277-498) fused to human IgG1 Fc at its C-terminus, consists of 721 amino acids, has a calculated molecular weight of 82 kDa, and a predicted N-terminus of His16 after signal peptide cleavage.

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1. Brindle, N.P.J., Saharinen, P., and Alitalo, K. Signaling and functions of angiopoietin-1 in vascular protection. Circ. Res. 98(8), 1014-1023 (2006).

2. Huang, H., Bhat, A., Woodnutt, G., et al. Targeting the ANGPT-TIE2 pathway in malignancy. Nat. Rev. Cancer 10(8), 575-585 (2010).

3. Bafunno, V., Firinu, D., D'Apolito, M., et al. Mutation of the angiopoietin-1 gene (ANGPT1) associates with a new type of hereditary angioedema. J. Allergy Clin. Immunol. 141(3), 1009-1017 (2018).

4. Hong, S., Jung, H.I., Ahn, T.S., et al. Expressions and clinical significances of angiopoietin-1, angiopoietin-2, and Tie-2 receptor in patients with colorectal cancer. Ann. Coloproctol. 33(1), 9-15 (2017).