Active • Host: HEK293 cells • AA: 19-496 • Tag: C-terminal IgG1 Fc • MW: 81.6 kDa
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Angiopoietin-2 (human, recombinant)

Item No. 42771

Product Insert (PDF)Safety Data Sheet (SDS) (PDF)
Technical Information
Synonyms
  • ANG-2
  • ANGP2
  • ANGPT2
Purity
≥90% estimated by SDS-PAGE
Endotoxin Testing
<1.0 EU/μg, determined by the LAL endotoxin assay
Source
Active recombinant C-terminal human IgG1 Fc-tagged ANG-2 expressed in HEK293 cells
Amino Acids
19-496
MW
81.6 kDa
Formulation (Request formulation change)
Lyophilized from sterile 20 mM MOPS, 150 mM sodium chloride, 0.20% CHAPS, pH 7.0, 5% Trehalose, 5% Mannitol, 0.01% Tween-80
Specific Activity
batch specific
UniProt Accession №
O15123
Shipping & Storage Information
Storage
-80°C
Shipping
Dry ice in continental US; may vary elsewhere
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    50 µg

    $218

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    500 µg

    $1,416

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    Cayman Chemical
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    Product Description

    Angiopoietin-2 (ANG-2) is a member of the angiopoietin family of growth factors.1,2,3 It is composed of a superclustering domain, which mediates oligomerization, a coiled-coil domain, and a fibrinogen-like domain, which facilitates receptor binding. ANG-2 forms homomultimers, which are required for binding to its receptor tunica interna endothelial cell kinase 2 (Tie2), where it acts as an agonist or antagonist in a context-dependent manner. It is expressed in endothelium in tissues undergoing vascular remodeling in response to inflammation and hypoxia.2,3 ANG-2 plays a role in vascular remodeling and permeability, angiogenesis, lymphangiogenesis, and embryonic development. Serum levels of ANG-2 are increased in various diseases with increased vascular permeability, including sepsis, post-cardiac arrest syndrome, acute lung or kidney injury, and acute liver failure.4 Increased protein levels of ANG-2 are associated with lymph node metastasis and decreased survival in patients with colorectal cancer.5 Cayman’s Angiopoietin-2 (human, recombinant) protein can be used for binding assays. This protein is a disulfide-linked homodimer. The reduced monomer, composed of ANG-2 (amino acids 19-496) fused to human IgG1 Fc at its C-terminus, consists of 716 amino acids, has a calculated molecular weight of 81.6 kDa, and a predicted N-terminus of Tyr19 after signal peptide cleavage.

    WARNING This product is not for human or veterinary use.

    References & Product Citations
    Product Description References

    1. Brindle, N.P.J., Saharinen, P., and Alitalo, K. Signaling and functions of angiopoietin-1 in vascular protection. Circ. Res. 98(8), 1014-1023 (2006).

    2. Huang, H., Bhat, A., Woodnutt, G., et al. Targeting the ANGPT-TIE2 pathway in malignancy. Nat. Rev. Cancer 10(8), 575-585 (2010).

    3. Akwii, R.G., Sajib, M.S., Zahra, F.T., et al. Role of angiopoietin-2 in vascular physiology and pathophysiology. Cells 8(5), 471 (2019).

    4. Eklund, L., and Saharinen, P. Angiopoietin signaling in the vasculature. Exp. Cell Res. 319(9), 1271-1280 (2013).

    5. Hong, S., Jung, H.I., Ahn, T.S., et al. Expressions and clinical significances of angiopoietin-1, angiopoietin-2, and Tie-2 receptor in patients with colorectal cancer. Ann. Coloproctol. 33(1), 9-15 (2017).