For immunochemical detection of H4K12Me1
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Histone H4K12Me1 Rabbit Monoclonal Antibody (Clone RM458)

Item No. 42793

Product Insert (PDF)
Technical Information
Synonyms
  • Monomethylated Histone H4 Lysine 12
Immunogen
A peptide corresponding to H4K12Me1
Clone Designation
RM458
MW
15 kDa
Formulation (Request formulation change)
100 µg of protein A-affinity purified monoclonal antibody
Storage Buffer
PBS with 50% glycerol, 1% BSA, and 0.09% sodium azide
Host
Rabbit
Isotype
IgG
Applications
ELISA, Multiplex-based assay, WB
Cross Reactivity
(+) H4K12Me1(-) Unmodified H4K12(-) Unmodified H4K5Me1(-) Unmodified H4K20Me1
Species Reactivity
(+) Human(+) Vertebrates
Shipping & Storage Information
Storage
-20°C
Shipping
Wet ice in continental US; may vary elsewhere
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Certificates of Analysis & Batch Specific Data

Provide batch numbers separated by commas to download or request available product inserts, QC sheets, certificates of analysis, data packs, and GC-MS data.

    100 µg

    $500

    Add
    Cayman Chemical
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    Product Description

    Histone H4 is one of four core histone proteins that are involved in the organization of DNA into chromatin.1 Histones are globular proteins with unstructured N-terminal tails and are subject to a variety of post-translational modifications, such as methylation, acetylation, phosphorylation, and citrullination, that can influence chromatin structure and regulate gene transcription.1,2 Histone H4 can be monomethylated at lysine 12 (H4K12Me1) by Set5, which is involved in cell growth and the stress response in yeast.3 H4K12 is also monomethylated by N6-adenine-specific DNA methyltransferase 1 (N6AMT1) and SET domain-containing protein 6 (SETD6) in prostate cancer cells in vitro.4,5 Cayman’s Histone H4K12Me1 Rabbit Monoclonal Antibody (Clone RM458) can be used for ELISA, multiplex-based assay, and Western blot (WB) applications. The antibody recognizes H4K12Me1 at 15 kDa from human and other vertebrate samples.

    WARNING This product is not for human or veterinary use.

    References & Product Citations
    Product Description References

    1. Wang, Y., Li, M., Stadler, S., et al. Histone hypercitrullination mediates chromatin decondensation and neutrophil extracellular trap formation. J. Cell Biol. 184(2), 205-213 (2009).

    2. Hyun, K., Jeon, J., Park, K., et al. Writing, erasing and reading histone lysine methylations. Exp. Mol. Med. 49(4), e324 (2017).

    3. Green, E.M., Mas, G., Young, N.L., et al. Methylation of H4 lysines 5, 8 and 12 by yeast Set5 calibrates chromatin stress responses. Nat. Struct. Mol. Biol. 19(3), 361-363 (2012).

    4. Metzger, E., Wang, S., Urban, S., et al. KMT9 monomethylates histone H4 lysine 12 and controls proliferation of prostate cancer cells. Nat. Struct. Mol. Biol. 26(5), 361-371 (2019).

    5. Weirich, S., Ulu, G.T., Chandrasekaran, T.T., et al. Distinct specificities of the HEMK2 protein methyltransferase in methylation of glutamine and lysine residues. Protein Sci. 33(2), e4897 (2024).